摘要
采用HPLC法测定不同产地生姜中姜酚类成分的含量,采用Agilent ZORBAX-SB C_(18)色谱柱(250 mm×4.6 mm,5μm),以乙腈-0.1%甲酸溶液为流动相进行梯度洗脱,流速1.0 mL/min,柱温35℃,检测波长282 nm,用标准曲线法分别对6-姜辣素、8-姜酚和10-姜酚定量.结果显示,6-姜辣素、8-姜酚和10-姜酚分别在1.005~100.5μg/mL(r=1)、1.001~100.1μg/mL(r=0.999 9)和0.996 5~99.65μg/mL(r=0.999 9)范围内线性关系良好;平均回收率(n=6)分别为99.31%(RSD 1.26%)、98.69%(RSD 1.48%)、98.55%(RSD 1.44%).测定的14批不同产地的生姜及不同部位,6-姜辣素、8-姜酚和10-姜酚含量存在一定的差异.由此认为所建立的方法准确、可靠、重现性好,能快速有效地测定生姜及不同部位的含量.
We established a HPLC method for content determination of gingerols in fresh ginger from different regions. Using the Agilent ZORBAX-SB C_（18） column（250 mm×4.6 mm,5 μm）and acetonitrile-0.1% methanoic acid solution as mobile phase by gradient elution,we set the flow rate at 1.0 mL/min,the temperature at 35 ℃ and the wavelength was 282 nm and directly calculated the quantity of 6-gingerol,8-gingerol and 10-gingerol in samples by standard curve method respectively. The results showed that 6-gingerol,8-gingerol and 10-gingerol had the good linearity at the range from 1.005-100.5 μg/mL（r=1）,1.001-100.1 μg/mL（r=0.999 9）and 0.996 5-99.65 μg/mL （r =0.999 9）,respectively. The average recoveries were 99.31%（RSD 1.26%）,98.69%（RSD 1.48%）and 98.55% （RSD 1.44%）for each. We determined 14 batches about the fresh ginger and the different parts and found that the contents of 6-gingerol,8-gingerol and 10-gingerol in different batches existed differences. The method was accurate,repeatable and rapid which was suitable for content determination of the fresh ginger and the different parts.
作者
宁二娟
李建
王韬
王学方
李晓
NING Erjuan;LI Jian;WANG Tao;WANG Xuefang;LI Xiao(Biological Developing Center of Henan, Henan Academy of Sciences, Zhengzhou 450002, Chin)
出处
《河南科学》
2018年第4期519-523,共5页
Henan Science
基金
河南省科技攻关项目(152102110115)
