摘要
目的探讨电针深刺法对三叉神经痛(TN)大鼠三叉神经节中P2Y2受体及Kv3.4表达情况的影响,进而阐明此法镇痛机制。方法将30只SD大鼠(成年健康雄性)随机分成假手术组、模型组、电针组,各10只。制备大鼠三叉神经痛模型(眶下神经慢性缩窄环术法)。3组均用细丝法检测大鼠痛阈,分别于14、28 d后检测痛阈并进行组间比较;3组均采用Western blot方法检测大鼠神经节(TG)神经元中P2Y2受体表达情况,并进行组间比较;3组均采用实时定量PCR技术检测大鼠神经节中Kv3.4 mRNA表达情况,并进行组间比较。结果电针深刺法治疗后三叉神经痛大鼠痛阈显著提高(P<0.05),TG中P2Y2受体的表达显著下降、Kv3.4 mRNA表达显著增加,与模型组比较差异有统计学意义(P<0.05),与假手术组比较差异无统计学意义(P>0.05)。结论此法可以通过降低P2Y2受体表达同时增加Kv3.4 mRNA表达,抑制神经元兴奋性而起到镇痛作用。
Objective To investigate the effect of electroacupuncture deep needle therapy on the expression of P2Y2 receptor and Kv3.4 in trigeminal neuralgia rats and to elucidate the analgesic mechanism of this method.Methods 30 SD rats（adult healthy male）were randomly divided into sham operation group,model group and electroacupuncture group,10 rats in each group.Preparation of rat trigeminal neuralgia model（infraorbital nerve chronic narrowing ring method）.The three groups were detected by the fi lament method of pain threshold,respectively,14 days and 28 days after the detection of pain threshold and compared between groups;Western blot was used to detect the expression of P2Y2 receptor in rat ganglioside（TG）neurons in all three groups and compared among groups;Three groups were used real-time quantitative PCR technology to detect the expression of Kv3.4 mRNA in rat ganglia,and compared between groups.Results The pain threshold of rats with trigeminal neuralgia increased significantly（P〈0.05）after EA acupuncture treatment,the expression of P2Y2 receptor was significantly decreased and the expression of Kv3.4 mRNA was significantly increased in TG group compared with model group（P〉0.05））,there are no significant difference compared with the sham operation group（P〉0.05）.Conclusion This method can reduce the P2Y2 receptor expression while increasing Kv3.4 mRNA expression,inhibition of neuronal excitability and play a role in analgesia.
作者
李崖雪
高瑞雪
刘潇
王丰
李晓陵
闫禹竹
王欣波
孙士红
任佰亮
LI Yaxue;GAO Ruixue;LIU Xiao;WANG Feng;LI Xiaoling;YAN Yuzhu;WANG Xinbo;SUN Shihong;REN Bailiang(The First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin 150040, China;Heilongjiang University of Chinese Medicine, Harbin 150040, China)
出处
《长春中医药大学学报》
2018年第2期221-223,共3页
Journal of Changchun University of Chinese Medicine
基金
黑龙江省自然科学基金面上项目(H2015033)
