摘要
目的:探讨二氧化铈纳米颗粒(nanoceria)预处理对大鼠缺血再灌注(IR)心肌细胞中核因子E2相关因子2(Nrf2)信号通路表达的影响。方法:选取健康成年大鼠50只,随机均分为5组:假手术组、模型组、nanoceria预处理1~3组。模型组、nanoceria预处理1~3组制备心肌IR动物模型(心肌缺血45 min后再灌注120 min),nanoceria预处理1~3组分别于造模术前24 h经尾静脉注射1~10、10~25和50 nm粒径的nanoceria悬液(2 m L/kg)。再灌注结束后,荧光定量PCR法检测再灌注区域心肌组织中Nrf2、醌氧化还原酶1(NQO1)及血红素氧化酶1(HO-1)mRNA表达水平,Western blot法检测胞核内Nrf2蛋白表达水平。结果:模型组心肌细胞内NQO1、HO-1 mRNA表达水平及胞核内Nrf2蛋白表达水平较假手术组增加(P<0.05),Nrf2 mRNA表达量无明显变化(P>0.05)。与模型组比较,nanoceria预处理1~3组Nrf2、NQO1和HO-1 mRNA及胞核内Nrf2蛋白表达水平增加(P<0.05),nanoceria预处理2组变化最显著(P<0.05)。结论:nanoceria预处理可能通过激活Nrf2信号通路,发挥心肌保护作用。
Aim：To research the influence of pretreatment of nanoceria on the expression of nuclear factor related factor 2（Nrf2）in cardiomyocytes of rats with ischemia-reperfusion（IR）injury.Methods：Fifty adult healthy rats were randomly allocated into 5 groups,and 10 in each group：the sham operation group,model group,and 3 pretreatment groups.The rats in model group and 3 pretreatment groups were made myocardial IR model（ischemia for 45 min and reperfusion for 120 min）.Pretreatment group 1,2,3 were injected through the tail vein with 1-10,10-25,and 50 nm nanoceria suspension（2 mL/kg）24 h before the operation.After the reperfusion,the expressions of Nrf2,NQO1 and HO-1 mRNA and the expression of Nrf2 protein in the nucleus in IR cardiomyocytes were detected by qRT-PCR and Western blot.Results：The expressions of NQO1,HO-1 mRNA,and Nrf2 protein in the nuclei in the model group was significantly higher than those in the sham operation group（P〈0.05）,but the expression of Nrf2 mRNA had no significant change（P〉0.05）.Compared with those of the model group,the expressions of Nrf2,NQO1,HO-1 mRNA and Nrf2 protein in the nuclei in the 3 pretreatment groups increased（P〈0.05）,and the changes of the above indexes in pretreatment group 2 were the most significant（P〈0.05）.Conclusion：The pretreatment with nanoceria may activate the Nrf2 signaling pathway,so as to achieve myocardial protection.
作者
黄真锋
法宪恩
朱方涛
王宏山
高成山
柳兵
HUANG Zhenfeng;FA Xian＇en;ZHU Fangtao;WANG Hongshan;GAO Chengshan;LIU Bing(Department of Cardiovascular Surgery,the Second Affiliated Hospital,Zhengzhou University,Zhengzhou 450014;Department of Cardiovascular Surgery,Zhengzhou NO.7 People＇s Hospital,Zhengzhou 450000)
出处
《郑州大学学报（医学版）》
CAS
北大核心
2018年第2期206-209,共4页
Journal of Zhengzhou University(Medical Sciences)
基金
国家自然科学基金资助项目(81241003)
河南省科技创新杰出人才计划资助项目(104200510007)
郑州市科技创新人才培育计划项目领军人才基金资助项目(10LJRC175)
