摘要
为探究铜藻多糖在葡聚糖酶作用下最佳的酶解条件及其不同分子量多糖组分产物的肠道环境调节功能,本试验采用响应面分析法优化铜藻多糖(SHP)酶解工艺条件,并考察所得多糖组分对小鼠肠道环境指标的影响。结果表明,SHP最优酶解工艺参数为酶解温度48℃、酶液浓度3.6%、pH值4.8、酶解时间2 h,测得的还原糖含量为0.550 mg·mL^(-1),与理论值基本吻合。SHP的酶解产物(SHPE)经分离纯化得到SHPE1、SHPE2、SHPE3 3种多糖组分,SHP纯化得到SHP0。对上述铜藻组分进行小鼠肠道环境调节功能试验,结果表明铜藻多糖组分均能够改善小鼠肠道环境,其中较低分子量的SHPE2和SHPE3与阳性对照组低聚果糖(FOS)在小鼠肠道pH值、内容物挥发性盐基氮(TVB-N)含量等肠道环境指标上相近。本研究表明铜藻多糖组分具备开发成为海洋藻类来源的益生元的潜力。
In order to investigate the optimum enzymatic hydrolysis conditions of Sargassum horueri polysaccharide（ SHP）under the action of polysaccharide enzyme and the regulation function of intestinal environment of polysaccharide components with different average molecular weight,response surface methodology was used to optimize the enzymatic hydrolysis process of SHP,as well as to investigate the effect of polysaccharide on the intestinal environment of mice.The results showed that the optimal enzymatic hydrolysis parameters were determined as follows： enzymolysis temperature48℃,enzyme concentration 3. 6%,pH value 4. 8,enzymolysis time 2 h. Under these conditions,the content of reducing sugars was 0. 550 mg ·mL^-1,which was consistent with the theoretical value. SHPE1,SHPE2,SHPE3 were obtained by the purification of Sargassum horueri polysaccharide enzymatic hydrolysates（ SHPE）,and SHP0 was obtained from SHP purified by column chromatography system. The intestinal microflora group regulating function test showed that the Sargassum horueri polysaccharide components exhibited a certain intestinal probiotic effect. Among them,the effects of SHPE2 and SHPE3 with relative low molecular weight on the intestinal pH value,the total volatile basic nitrogen（ TVB-N） content and other intestinal probiotics indicators in mice were close to fructooligosaccharides（ FOS）. This study informed that Sargassum horueri polysaccharide components had significant potential to be developed into a marine algae prebiotics.
作者
严志鹏
张皓雅
涂红艳
陈晓晓
邵平
YAN Zhipeng;ZHANG Haoya;TU Hongyan;CHEN Xiaoxiao;SHAO Ping(Zhejiang University of Technology, Department of Food Science and Engineering, Hangzhou, ZheJiang 310014;Comprehensive Technical Service Center of the Huzhou Inspection and Quarantine Bureau, Huzhou, Zhcjiang 313000)
出处
《核农学报》
CAS
CSCD
北大核心
2018年第6期1153-1161,共9页
Journal of Nuclear Agricultural Sciences
基金
国家自然科学基金(31301560)
关键词
铜藻
多糖
酶解
益生元
Sargassum horueri, polysaccharides, enzymolysis, prebiotics
