摘要
目的:改良建立Ph^+急性淋巴细胞白血病(ALL)小鼠模型的方法,为Ph^+ALL提供更加便捷的研究工具。方法:使用Mig190逆转录病毒转染BABL/c小鼠前体B细胞,输注同系小鼠,建立Ph^+ALL模型;应用外周血涂片,流式细胞术鉴定免疫表型,RT-PCR和Western blot方法鉴定BCR-ABL转录和蛋白表达,并分离发病小鼠的白血病细胞进行再次输注。结果:Mig190逆转录病毒转染后小鼠发生急性淋巴细胞白血病,在血涂片观察到原始和幼稚淋巴细胞,免疫分型示白血病细胞CD19+;RT-PCR和Western blot均检测到BCR-ABL,符合Ph^+ALL的免疫表型和分子生物学特征;再次输注未经照射的同系小鼠能迅速发生B-ALL。结论:改良建立的Ph^+ALL小鼠模型,能够为研究Ph^+ALL提供更简便而有效的工具。
Objective： To investigate a modified protocol of establishing mouse Ph ＋ ALL model so as to provide a more convenient and more powerful tool for Ph ＋ ALL studies. Methods： Immature B cells from BALB/c mice were transfected with the Migl90 retrovirus and infused into irradiated syngeneic mice. The immunophenotype was identified by flow cytometry, the BCR-ABL was identified by RT-PCR and Western blot. Leukemia cells isolated from sick mice were re-infused into syngeneic mice without irradiation. Results： The acute lymphoblastic leukemia was established in mice after Migl90 retroviral transfection, the lymphoblasts were observed by blood smears, the immunophenotype of these leukemic cells was CD19 ＋ , and the BCR-ABL was detected by RT-PCR and Western blot, the immunophenotype was conformed as Ph ＋ ALL, the isolated leukemia cells infused into mice rapidly developed B-ALL. Conclusion： A new protocol is established to generate mice with Ph ＋ ALL. This mouse model can provide a more simple and effective tool in comparison with the conversional protocols.
作者
祁娜
王雪
吴庆运
闫志凌
王林
陈翀
徐开林
QI Na1 , WANG Xue2, WU Qing-Yun2 ,YAN Zhi-Ling1, WANG Lin1 , CHEN Chong1 ,XU Kai-Lin1,2(1 Institute of Hematolog, Xuzhou Medical University, 2 Department of Hematology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou 221002, Jiangsu Province Chin)
出处
《中国实验血液学杂志》
CAS
CSCD
北大核心
2018年第2期341-346,共6页
Journal of Experimental Hematology
基金
国家自然科学基金(81471580
81300399)
江苏省自然科学基金(BK20141138)
