摘要
目的:研究不同浓度脱落酸(Abscisic acid,ABA)对雷公藤悬浮细胞中萜类次生代谢产物的诱导效应,探讨脱落酸的毒物效应(Hormesis)。方法:雷公藤悬浮细胞继代培养10 d后,分别加入不同浓度(10μM,20μM,50μM,100μM,150μM,200μM,500μM)的ABA诱导,并设置空白对照组,每组生物学重复4次。分别采用HPLC和UPLC检测诱导后0 h和240 h的样品,测定悬浮细胞中雷公藤甲素、雷酚内酯、雷公藤红素和雷公藤内酯甲4种萜类成分的含量。结果:当ABA浓度为10μM和20μM时,诱导组与对照组无差异;50μM时,与对照组比较,雷酚内酯含量明显升高,雷公藤红素含量升高至2.4倍,雷公藤内酯甲含量升高至4.7倍;当诱导浓度超过100μM时,与对照组比较,除雷公藤内酯甲外,雷公藤甲素、雷酚内酯、雷公藤红素含量均显著降低。结论:ABA在10~500μM范围内对不同的雷公藤萜类成分影响作用不同,从整体趋势看,呈现为β曲线,符合Hormesis效应特点;50μM时ABA对雷公藤红素含量的积累有显著促进作用,为后期研究ABA影响雷公藤红素合成机制奠定基础。
Objective： To investigate the induction effect of abscisic acid（ ABA） on the terpenoid secondary metabolites in the suspension cells of Tripterygium wilfordii,and to explore the hormesis of abscisic acid. Methods： After being subcultured for 10 days,the suspension cells of Tripterygium wilfordii were induced by ABA at different concentrations（ 10 μM,20 μM,50 μM,100μM,150 μM,200 μM,500 μM）,and the blank control group was set for contrast. They were repeated for 4 times. The HPLC and UPLC was used to detect the samples at 0 h and 240 h after induction,respectively,and the contents of triptolide,triptophenolide,celastrol and wilforlide A were determined in suspension cells. Results： When the concentration of ABA was 10 μM and 20 μM,there was no difference between the induction group and the control group. At 50 μM,the content of triptophenolide significantly increased and the content of celastrol increased to 2. 4 times,compared with the control group. And the content of wilforlide A increased to 4. 7 times; when the induction concentration exceeded 100 μM,compared with the control group,except wilforlide A,content of triptolide,triptophenolide and celastrol were significantly lower. Conclusion： The effect of ABA on different terpenoids of Tripterygium wilfordii differs from 10 μM to 500 μM. From the overall trend,ABA shows a β-curve,which is in line with the hormesis effect. The 50 μM of ABA significantly enhances the accumulation of celastrol,which laid the foundation for later study on the mechanism of ABA affecting tripterine synthesis.
作者
张睿
吴晓毅
马宝伟
陈上
王秀娟
高伟
黄璐琦
Zhang Rui;Wu Xiaoyi;Ma Baowei;Chen Shang;Wang Xiujuan;Gao Wei;Huang Luqi(School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China;National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China)
出处
《世界中医药》
CAS
2018年第2期264-270,共7页
World Chinese Medicine
基金
"十三五"时期北京市属高校高水平教师队伍建设支持计划(CIT&TCD20170324)
市教委科技+社科计划重点项目(KZ201710025022)
国家自然科学基金面上项目(81773830)
