摘要
目的探讨高碘染毒对雄性子代大鼠学习记忆及海马神经细胞自噬的影响。方法将24只SPF级雌性SD孕鼠随机分为4组,分别为对照(自来水)组及500、2 500、5 000μg/L碘酸钾染毒组,每组6只。自妊娠至仔鼠出生后第21天,孕鼠经自由饮水方式进行染毒。断乳后,将雄性仔鼠继续按与母鼠相同的染毒处理,直至第六个月末。采用Morris水迷宫实验观察雄性仔鼠学习记忆能力,Western Blot法检测雄性仔鼠海马神经细胞自噬相关蛋白的表达。结果在定位航行实验中,与对照组比较,各碘酸钾染毒组大鼠到达平台的潜伏期均延长,仅5 000μg/L碘酸钾染毒组大鼠到达平台的总路径较长,差异均有统计学意义(P<0.05);而各组大鼠的游泳速率无明显改变。且随着碘酸钾染毒浓度的升高,仔鼠到达平台潜伏期、总路径均呈延长趋势。在空间探索实验中,与对照组比较,各碘酸钾染毒组大鼠水迷宫空间探索时间比例均下降,2 500、5 000μg/L碘酸钾染毒组路径比例均下降,差异有统计学意义(P<0.05);且随着碘酸钾染毒浓度的升高,仔鼠水迷宫空间探索时间、路径比例均呈下降趋势。与对照组比较,2 500、5 000μg/L碘酸钾染毒组雄性仔鼠海马神经细胞LC3-Ⅱ、Beclin1和p62蛋白的表达水平均较高,差异有统计学意义(P<0.05);且随着碘酸钾染毒浓度的升高,雄性仔鼠海马神经细胞LC3-Ⅱ、Beclin1的表达水平均呈上升趋势。结论高碘可损伤雄性子代大鼠的学习记忆能力,并且引起雄性子代大鼠海马神经细胞自噬的改变。
Objective To understand the effects of higher iodine in water on learning and memory and autophagy of hippocampal neurons in male offspring rats. Methods A total of 24 SPF female SD pregnant rats were randomly divided into four groups, six in each,the control （tap water） group,500,2 500 and 5 000μg/L potassium iodate exposure group. From pregnancy to the 21st day after the birth of pups,the pregnant rats were exposed to potassium iodate through drinking water. After weaning,the male offspring rats were continued to be treated with the same exposure as the parent did until the end of the sixth month. Morris water maze was used to observe the learning and memory ability of male offspring rats. The expression of autophagy-related protein in hippocampus was detected by Western blot. Results In the place navigation experiment, compared with the control group,the latency of all the rats in potassium iodate group were longer,and only in 5 000μg/L potassium iodate group,the rats experienced a longer total path （P〈0.05）. The swimming speed of rats in each group had no significant change. And with the increase of potassium iodate exposure concentration,the latency and the total path showed an extension trend. In the space exploration experiment, compared with the control group,the spatial probe time of rats in each potassium iodate group decreased, the path proportion in 2 500 and 5 000μg/L potassium iodate groups significantly decreased （P〈0.05）. And with the increase of potassium iodate concentration,the space probe time and route of offspring showed a decreasing trend. Compared with the control group,the expression levels of LC3-Ⅱ ,Beclinl and p62 in hippocampal neurons of 2 500 and 5 000μg/L potassium iodate exposure groups were higher than those of the control group （P〈0.05）. And with the increase of potassium iodate concentration,the expression levels of LC3-Ⅱ and Beclinl in hippocampal neurons of male offspring all showed an increasing trend. Conclusion Higher iodine in water can damage
作者
聂珺妍
张斌
赵阳
张祖山
虞静雯
崔玉山
赵亮
刘洪亮
NIE Jun-yan;ZHANG Bin;ZHAO Yang;ZHANG Zu-shan;YU Jing-wen;CUI Yu-shan;ZHAO Liang;LIU Hong-liang(College of Public Health, Tianjin Medical University, Tianjin 300070, Chin)
出处
《环境与健康杂志》
CAS
北大核心
2018年第1期1-5,共5页
Journal of Environment and Health
基金
国家自然科学基金(81372934
81573107)
天津市卫生系统科技攻关项目(14KG120)
天津市疾病预防控制中心科研项目(CDCKY1501)
关键词
高碘
饮用水
自噬
学习
记忆
Higher iodine
Drinking water
Autophagy
Learning
Memory
