摘要
目的测定排草香不同溶剂提取物对人胃癌细胞MGC-803细胞毒性的影响及其中木犀草素的含量。方法分别采用四甲基偶氮唑盐(MTT)及磺酰罗丹明B(Sulforhodamine B,SRB)比色法测定排草香不同溶剂(水、乙醇、乙醚)提取物对人胃癌细胞MGC-803的细胞毒性,以紫杉醇组作为阳性对照;建立高效液相色谱(High performance liquid chromatography,HPLC)法测定不同提取物中木犀草素的含量。结果 MTT、SRB法测定显示,排草香各提取物组及紫杉醇组均对MGC-803细胞显示出一定的毒性;IC50对比各组细胞毒性:紫杉醇组>醚提物组>醇提物组>水提物组,且排草香醚提物组及醇提物组的细胞毒性均显著高于水提物组(P<0.05);木犀草素在0.03~0.77 mg/m L范围内线性关系良好(r=0.999 9,n=7),仪器精密度良好(RSD=0.17%,n=6),重复性良好(RSD=0.32%,n=6),加样回收率良好(101.13%,RSD=1.43%,n=6),稳定性良好(RSD=0.16%,n=6);各提取物中木犀草素含量对比:醚提物(0.98%)>醇提物(0.65%)>水提物(0.43%)。结论排草香具有一定的抗癌活性,且其活性可能与木犀草素含量有关,为临床排草香抗癌的应用提供理论依据。
Objective To explore the influence of different solvents extracted from lysimachia on cytotoxicity of human gastric cancer cell line MGC-803 and to determine the content of luteolin. Methods Extracts of different solvents （water, ethyl alcohol and ether） on cytotoxicity of human gastric cancer cell line MGC-803 was detected by u- sing four methyl pyrazole （MTT） salt and sulforhodamine B （ SRB ） colorimetric method ; the paclitaxel group was used as the positive control. The content of luteolin in different extracts was detected by establishing high performance liquid chromatography （HPLC） method. Results Results of MTT and SRB methods showed that the extracts groups and paclitaxel group expressed certain toxicity on MGC-803 cell. Cytotoxicity of each group （ compared by IC50 ） ： taxol group 〉 ether extract group 〉 ethanol extract group 〉 water extract group, and cell toxicity of ether extract group and ethanol extract group was significantly higher than that of water extract group （ P 〈 0. 05 ）. Luteolin in 0. 03 - 0. 77 mg/ mL showed good linear relationship （ r = 0. 999 9, n - 7 ） , with good precision （ RSD = 0. 17% , n - 6 ） , good reproduc- ibility （RSD -0. 32% ,n -6） ,good recovery rate of samples （mean value 101. 13% ,RSD -1.43% ,n -6） ,and good stability （ RSD - 0. 16% , n - 6 ）. The content of luteolin ： ether extract （ 0. 98 % ） 〉 ethanol extract （ 0. 65 % ） 〉 water extract （0.43 % ）. Conclusion Luteolin has certain anti-cancer activity, which may be related to the content of luteolin and provides theorectical evidence for its clinical application.
作者
杨芳
吴泽幼
包思
梁敬
YANG Fang;WU Ze-you;BAO Si;LIANG Jing(Department of Pharmacy, Nanfang Hospital of Southern Medical Universi- ty, Guangzhou 510515, China)
出处
《实用药物与临床》
CAS
2018年第4期419-424,共6页
Practical Pharmacy and Clinical Remedies
基金
广东省医学科学技术研究基金项目(A2014273)
关键词
排草香
细胞毒性
高效液相色谱法
木犀草素
Lysimachia
Cytotoxicity
High performance liquid chromatography
Luteolin
