摘要
对油茶籽多糖Ⅰ(Camellia Oleifera Seed PolysaccharideⅠ,CPSI)的分离纯化、单糖组分分析及其降脂作用进行研究。从油茶籽中提取水溶性粗多糖,采用DE-52纤维素柱层析、Sephadex G-100柱层析和亲和层析纯化得油茶籽多糖Ⅰ(CPSI),毛细管电泳法研究CPSI的单糖组分。使用高血脂小鼠模型,测定CPSⅠ对总胆固醇(T-CHO)、高密度脂蛋白胆固醇(HDLC)、低密度脂蛋白胆固醇(LDL-C)、3-羟基-3-甲基戊二酰辅酶A还原酶(HMG-Co A)和粪固醇的影响,研究CPSI的潜在降脂机制。油茶籽多糖Ⅰ(CPSI)为具有多种磷酸基团的复合多糖。CPSⅠ由木糖、半乳糖组成,CPSⅠ水解液中半乳糖和木糖的摩尔比为4.6∶5.57。与高血脂组相比,CPSI处理组小鼠血清T-CHO和LDL-C显著下降,粪固醇摩尔质量浓度升高,但HDL-C和HMG-Co A还原酶活性没有变化,CPSI对预防小鼠高血脂有一定疗效,其可能在降低心血管疾病风险方面发挥一定的作用。
The purification,components analysis and hypolipidemic activity of a polysaccharide from Camellia oleifera Abel. seeds （CPSI） were studied. The crude polysaccharides were extracted from the seeds of Camellia oleifera Abel. using deionized water. The Camellia oleifera seeds polysaccharides （CPS） were further separated and purified by anion exchange chromatograph on DE-52. Sephadex G-100 column and affinity chromatography. The monosaccharide composition in the Cmellin oleifera seed polysaccharide I was determined by capillary electrophoresis.The potential mechanisms underlying lipid-lowering affect of CPSI were examined,specifically alterations in levels of the total cholesterol （T-CHO）, High-density lipoprotein cholesterol （HDL-C）, low-density lipoprotein cholesterol （LDL-C）,3-hydroxy-3-methyl glutaryl coenzyme A reductase （HMG-CoA） and coprosterol,using a mice model of hypercholesterolemia. CPS I was conjugated polysaccharide which has many phosphate groups. The polysaccharide contained xylose and galactose, and the mole ratio of galactose and xylose was 4.6：5.57 in the hydrolytic solution of CPS I .Serum T-CHO, LDL-C levels in mice treated with CPSI declined significantly when compared to hyperlipid control group,and coprosterol of mice in the groups treated with CPSI was higher than that in hyperlipid control group.Serum HDL-cholesterol and HMG-CoA activity,however.was unchanged.These results indicate that CPSI is useful for the prevention of hyperlipidemi in mice and it may therefore play a valuable role in reducing cardiovascular risk.
作者
张宽朝
文汉
陶俊
王海林
ZHANG Kuanchao;WEN Han;TAO Jun;WA NG Hailin(Anhui Agricultural University, College of Life Science, Hefei, 230036, Chin)
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2018年第3期329-335,共7页
Journal of Food Science and Biotechnology
关键词
油茶籽
多糖
纯化
组分分析
降血脂
Camellia oleifera seed,polysaccharide,purification, components analysis,lipid-lowering effect
