摘要
以西藏地区4种不同生境的野生披碱草属植物作为试验材料进行干旱胁迫模拟盆栽试验,通过对比幼苗叶片中相对含水量(RWC)、丙二醛(MDA)、超氧化物歧化酶(SOD)、游离脯氨酸(Pro)、以及植物体内可溶性糖(SSC)含量的变化对供试幼苗进行抗旱性分析与评价。干旱处理4d、8d、12d、16d,测其叶片生理指标(RWC、MDA、Pro、SOD、SSC)。研究表明:4种披碱草属植物幼苗在干旱胁迫条件下,RWC含量随胁迫程度的加剧而下降,MDA、Pro及SSC含量随胁迫程度的加剧而上升,SOD则是先上升后下降。5个生理指标在4种披碱草属植物幼苗间均存在显著差异,可以作为披碱草属植物抗旱性的评价指标。在胁迫8d后各测试指标的变化趋势均出现了明显的转折点,表明8d后土壤的含水量可能是披碱草属幼苗耐旱的关键水势点。本试验采用隶属函数法对4种西藏野生披碱草属幼苗进行抗旱性综合评价后,得出其抗旱性强弱顺序依次为:申扎垂穗披碱草>那曲垂穗披碱草>林芝垂穗披碱草>拉萨老芒麦。
The seedlings of four kinds of different habitats of wild Elymus species from the Tibet were used in a potted experiment.The seedling drought stress tolerance was investigated by comparing the changing of relative water content（RWC）,malondialdehyde（MDA）,free proline（Pro）,superoxide dismutase（SOD）and soluble sugar content（SSC）in seedling leaves.We did four drought treatments and sampled at4 days,8 days,12 days and 16 days for observing physiological index of leaves（RWC,MDA,SOD,Pro and SSC）.The results revealed that the rate of RWC reduced with the stress extended.MDA,Pro and SSC increased with stress prolonged.SOD increased and then decreased for 4 types of Elymus.The physiological index had also significant difference,which can be as the index of drought stress evaluation for the filed Elymus.The trend of index revealed a turning point after drought stress of 8 days,which could be the critical soil water potential points of Elymus.This paper used the average subordinative function method to evaluate the 4 types of filed Elymus in Tibet and the rank of water stress tolerance was E.natans（Shengzha） E.natans（Naqu） E.natans（Linzhi） E.sibircus（Lasa）.
作者
闫天芳
苗彦军
王向涛
魏臻武
徐雅梅
蔺永和
YAN Tian-fang;MIAO Yan-jun;WANG Xiang-Tao;WEI Zheng-wu;XU Ya-mei;LIN Yong-he(Tibet Agricultural and Animal Husbandry College, Nyingchi 860000, China;College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225009, China;College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, Gansu 730020, China)
出处
《草地学报》
CAS
CSCD
北大核心
2017年第6期1333-1339,共7页
Acta Agrestia Sinica
基金
西藏自治区科技厅重点项目(2017ZDKJZC-15
2016KJTZD01)
国家重点研发计划项目(2016YFC0502002)
国家自然基金(41761008)资助
关键词
披碱草属
苗期
干旱胁迫
生理指标
西藏
Elymus
Seedling stage
Drought stress
Physiological index
Tibet
