摘要
以紫花苜蓿不同品种(‘三得利'‘新牧2'‘赛迪5'‘WL656'‘WL168'‘WL903'‘WL712'‘WL343')为试验材料,研究150g/L PEG-6000胁迫下,幼苗抗旱性与ABA受体基因表达的关系。结果表明,150g/L PEG处理下,不同苜蓿幼苗较对照根系缩短(10.58%~15.88%)、苗长减少(18.06%~23.71%)、鲜干质量比降低(24.51%~37.81%)、相对含水量下降(13.31%~16.71%),差异显著;脯氨酸质量分数均显著升高(21.24%~39.04%),抗旱性强弱为:‘WL168'>‘新牧2'>‘WL712'>‘赛迪5'>‘WL343'>‘WL656'>‘WL903'>‘三得利';‘WL168'的ABA受体Medtr1g016480和Medtr5g083270基因的表达量显著升高,二者在根茎叶中的表达量分别为6.10、1.79、3.04和6.51、1.67、1.89,其表达量强弱为:根>叶>茎。渗透胁迫下,苜蓿ABA受体Medtr1g016480和Medtr5g083270基因的表达量显著升高,对苜蓿生理生化反应及其生长起到一定的调节作用,进而影响苜蓿的抗旱能力。
Using alfalfa varieties ＇Sanditi＇ ＇Sardi5＇ ＇Xinmu2＇ ＇WL656＇ ＇WL168＇ ＇WL903＇ ＇WL712＇ and ＇WL343＇ as experimental materials, we studied the relationship between drought resistance and expression of abscisic acid receptor gene of alfalfa under stress of 150 g/L PEG-6000. The results showed that： under 150 g/L PEG stress, the root length （10. 58%- 15. 88%）, stem length （18.06%--23.71%）, fresh-dry ratio （24. 51%- 37. 81%）, relative water content （13. 31%- 16.71%） of the TM cultured alfalfa were significantly reduced compared with the CK cultured alfalfa; the proline mass fraction of the TM cultured alfalfa were significantly increased compared with the CK cultured alfalfa（21.24%--39.04%）. The results showed that,the drought resistance of alfalfa was as follows; ＇ WL168＇ 〉＇ Xinmu2 ＇ 〉＇ WL712 ＇〉＇ Sardi5 ＇〉＇ WL343 ＇〉＇ WL656 ＇〉＇ WL903 ＇ 〉＇ Sanditi ＇. Under osmotic stress, the expression of Medtr1gO16480 and MedtrSg083270 of abscisic acid receptor gene of ＇WL168＇ were higher than the others; the expression of the both in root, stem and leaf were 6.10, 1.79, 3.04 and 6.51, 1.67, 1.89. And the expression strength were as follows： root〉leaf〉 stem. Under osmotic stress, the expression ofMedtrlg016480 and MedtrSg083270 were significantly increased. It could affect the ability of drought resistance by regulating the reaction of physiology and biochemistry and the growth.
作者
薛泽民
薛琪
高景慧
XUE Zemin, XUE Qi and GAO Jinghui(College of Animal Science and Technology, Northwest A~F University, Yangling Shaanxi 712100, Chin)
出处
《西北农业学报》
CAS
CSCD
北大核心
2018年第3期331-338,共8页
Acta Agriculturae Boreali-occidentalis Sinica
基金
中央高校基本科研业务费(ZD2013006)
陕西省科技攻关(2012K01-38-01)~~
