摘要
目的探讨白细胞介素-27（IL-27）基因多态性与艾滋病易感性的关系。方法选取艾滋病患者和健康对照者各100例作为研究对象,运用多重单碱基延伸（SNaPshot SNP）技术检测IL-27基因rs17855750、rs181206、rs40837单核苷酸多态性,用SHEsis软件分析IL-27基因的连锁不平衡及单倍型频率,同时采用酶联免疫吸附试验（ELISA）测定IL-27血清水平,用χ^2检验和t检验进行统计学分析。结果艾滋病组和对照组IL-27基因rs17855750、rs181206、rs40837的基因型和等位基因的分布频率差异均无统计学意义（P〉0.05）。对照组和艾滋病组的单倍型分布频率提示,rs17855750、rs18126、rs40837这三个位点的AAG单倍型是艾滋病的一个保护因子[比值比（OR）=0.572,95%可信区间（CI）：0.330~0.990,P〈0.05]。携带rs181206A等位基因的艾滋病患者,其血清IL-27水平低于不携带者（P〈0.05）。结论 AAG单倍型可降低艾滋病的易感性,rs181206A等位基因携带可能与艾滋病危险性有关。
Objective To investigate the correlation between genotypes polymorphism of interluekin-27 and the susceptibility of AIDS.Methods By using the multiple single base extention（SNaPshot SNP）techniques,we analyzed IL-27 gene rs17855750,rs181206,and rs40837 single nucleotide polymorphisms of 100 AIDS patients and another 100 age and sex matched controls.The SHEsis is used to analyze the linkage disequilibrium and haplotypes of IL-27,and determine the serum level of IL-27 by ELISA.Statistical analysis was performed with χ^2 test and t test.Results The frequencies of IL-27 rs17855750,rs181206,rs40837 genotypes and alleles were not significantly different between AIDS and control groups（P〉0.05）.Consistent with the results of the genotyping analyses,frequency of AAG haplotype in patients with AIDS was lower than those in controls（P〈0.05,OR=0.572,95%CI=0.330-0.990）.The serum level of IL-27 rs181206 Aallele carriers was lower than that of no carriers in AIDS patients（P〈0.05）.Conclusion The haplotype AAG may reduce the susceptibility of AIDS,and the rs181206 Aallele may increase the susceptibility of AIDS.
作者
庞晓霞
覃海媚
王荣
唐毓金
李晓华
韦贵将
杨凤莲
胡仁统
王俊利
PANG Xiaoxia1, QIN Haimei1 , WANG Rong1 , TANG Yujin2 , LI Xiaohua1 , WEI Guijiang2 , YANG Fenglian1 , HU Rentong2 , WANG Jun li2.(1. Youjiang Medical University for Nationalities, Baise, 533000, China; 2. Affiliated Hospital of Youjiang Medical University for Nationalitie)
出处
《中国艾滋病性病》
CAS
CSCD
北大核心
2018年第3期223-226,共4页
Chinese Journal of Aids & STD
基金
2016年度百色市科学研究与技术开发计划(百科20160610)
