摘要
目的研究贵州苗药吉祥草Reineckia carnea中的皂苷类成分。方法用95%乙醇提取,萃取后利用常规硅胶柱、大孔树脂、ODS C18、Sephadex LH-20及Pre-HPLC等进行分离纯化,通过理化性质、HR-ESI-MS和核磁波谱进行结构鉴定。结果从吉祥草的正丁醇部位分离得到10个皂苷类化合物,分别鉴定为(1β,3β,16β,22S)-cholest-5-ene-1,3,16,22-tetrol-1,16-di-(β-D-glucopyranoside)(1)、(1β,3β,16β,22S)-cholest-5-ene-1,3,16,22-tetrol1-[O-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside]-16-(β-D-glucopyranoside)(2)、(20S,22R)-spirost-25(27)-en-1β,3β,4β,5β-tetraol-5-O-β-D-glucopyranoside(3)、kitigenin 5-O-β-Dglucopyranoside(4)、蜘蛛抱蛋苷A(5)、(17,20-S-trans)-5β-pregn-16-en-1β,3β-diol-20-one-1-O-β-D-xylopyranosyl-(1→2)-[α-L-rhamnopyranosyl]-3-O-α-L-rhamnopyranoside(6)、薯蓣皂苷元-3-O-β-D-吡喃葡萄糖苷(7)、麦冬皂苷T(8)、prosaikogenin D(9)、柴胡皂苷b2(10)结论化合物3、5、8~10均为首次从吉祥草中分离得到,为进一步阐明和开发吉祥草中皂苷类成分提供了一定的参考。
Objective To study the saponins from Reineckia carnea of Guizhou Miao medicine. Methods The chemical constituents from 95% Et OH extract were isolated and purified by repeated silica gel column chromatography, macroporous resin, ODS C18, Sephadex LH-20 gel column chromatography, and Pre-HPLC, and their structures were identified on the basis of physical and chemical properties, HR-ESI-MS and NMR spectral analysis. Results Ten compounds were isolated and identified as（1β,3β,16β,22 S）-cholest-5-ene-1,3,16,22-tetrol1,16-di（β-D-glucopyranosede）（1）,（1β,3β,16β,22 S）-cholest-5-ene-1,3,16,22-tetrol-1-[O-α-L-rhamnopyranosyl-（1→2）-β-D-glucopyranoside]-16-（β-D-glucopyranoside）（2）,（20 S,22 R）-spirost-25（27）-en-1β,3β,4β,5β-tetraol-5-O-β-Dglucopyranoside（3）, kitigenin 5-O-β-D-glucopyrannoside（4）, aspidistrin A（5）,（17,20-S-trans）-5β-pregn-16-en-1β,3β-diol-20-one-1-O-β-D-xylopyranosyl-（1→2）-[α-L-rhamnopyranosyl]-3-O-α-L-rhamnopyranoside（6）, diosgenin-3-O-β-D-glucopyranoside（7）, ophiopogonin T（8）, prosaikogenin D（9）, and saikosaponin b2（10）. Conclusion Compounds 3, 5, 8, 9, and 10 are isolated from this plant for the first time, which provided some reference for further elucidation and development of saponins from R. carnea.
作者
徐旭
吴蓓
李艳
詹艳芝
温泉
谭婷
冯育林
钟国跃
杨世林
XU Xu1, WU Bei2, LI Yan1, ZHAN Yan-zhi1, WEN Quan1, TAN Ting1, FENG Yu-lin1, ZHONG Guo-yue1, YANG Shi-lin3(1. Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China 2. Nanchang Food and Drug Inspection Office, Nanchang 330029, China 3. State Key Laboratory of Innovative Drug and Efficient Energy-Saving Pharmaceutical Equipment, Nanchang 330006, Chin)
出处
《中草药》
CAS
CSCD
北大核心
2018年第6期1261-1265,共5页
Chinese Traditional and Herbal Drugs
基金
国家自然科学青年基金项目(81603274)
江西省科技厅青年科学基金资助项目(2016 1BAB215189)
