摘要
目的研究细叶杜香Ledum palustre叶中的三萜类化学成分及其细胞毒活性。方法采用硅胶柱色谱、重结晶和HPLC等分离方法进行分离纯化,并经波谱数据鉴定结构。采用MTT法研究化合物的细胞毒活性。结果从细叶杜香叶中分离得到9个化合物,分别鉴定为2α-羟基-9(11)-羊齿烯-3-酮(1)、熊果醇(2)、羽扇豆酮(3)、α-香树脂酮(4)、熊果酸(5)、羽扇豆醇(6)、α-香树脂醇(7)、2α,3β-二羟基-9(11)-羊齿烯(8)、羊齿烯醇(9)。结论化合物1为新化合物,命名为杜香酮,化合物4、8和9系首次从细叶杜香中分离得到。细胞毒活性结果显示,羊齿烯型三萜化合物1、8、9对人非小细胞肺癌细胞株A549和人白血病细胞株K562无明显抑制作用。
Objective To study the chemical constituents from the leaves of Ledum palustre and their antitumor activities. MethodsThe chemical constituents were isolated and purified by chromatography of silica gel column, recrystallization and HPLC, and their structures were elucidated by spectral analysis. The in vitro cytotoxic activities of the isolated compounds were studied by MTT method. Results Nine compounds were isolated and identified as ledumone（1）, uvaol（2）, lepenone（3）, α-amyrenone（4）, ursolic acid（5）, lupeol（6）, α-amyrin（7）, fern-9（11）-ene-2α,3β-diol（8）, and fernenol（9）. Conclusion Compound 1 is new named as ledumone, and compounds 4, 8, and 9 are isolated from Ledum palustre for the first time. Fernane type triterpene 1, 8, and 9 show no significant cytotoxicities against A549 and K562 cells.
作者
张扩
赵明
王美娇
王丹
王金兰
张树军
ZHANG Kuo, ZHAO Ming, WANG Mei-jiao, WANG Dan, WANG Jin-lan, ZHANG Shu-jun(Institute of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar 161006, Chin)
出处
《中草药》
CAS
CSCD
北大核心
2018年第6期1250-1254,共5页
Chinese Traditional and Herbal Drugs
基金
黑龙江省自然科学基金面上项目(B201209)
