摘要
目的探讨哺乳期高水平蛋白质供给对早产大鼠神经系统发育的影响及其机制。方法采用SPF级SD大鼠,足月组及早产组仔鼠均母乳喂养,母鼠哺乳期予以高蛋白饲料或标准蛋白饲料,分为早产高蛋白组、早产标准蛋白组、足月高蛋白组和足月标准蛋白组,每组16只仔鼠。从第22天起哺乳期结束,各组仔鼠均予标准蛋白饲料喂养。仔鼠喂养至6周或8周,采用Morris水迷宫行定位航行实验,记录逃避潜伏期。定位航行实验结束后,采用免疫组织化学方法检测脑组织海马区哺乳动物雷帕霉素靶蛋白(mammalian target of rapamycin,m TOR)阳性细胞表达。采用方差分析、t检验进行统计分析。结果 (1)6周龄:随训练天数的延长,各组大鼠的逃避潜伏期均逐渐缩短。第4天,早产标准蛋白组的逃避潜伏期高于足月标准蛋白组[(40.5±11.9)与(28.3±10.2)秒],海马CA1区m TOR阳性细胞数量低于足月标准蛋白组[(35.2±2.9)与(45.7±6.5)个](P值均﹤0.05)。虽然早产高蛋白组的逃避潜伏期低于早产标准蛋白组,但差异无统计学意义[(34.4±12.8)与(40.5±11.9)秒,P﹥0.05]。早产高蛋白组与足月高蛋白组的逃避潜伏期[(34.4±12.8)与(28.1±16.4)秒]、m TOR阳性细胞数量[(36.1±3.7)与(39.4±1.7)个]比较,差异无统计学意义(P值均﹥0.05)。(2)8周龄:第4天的逃避潜伏期,早产高蛋白组与标准蛋白组比较[(29.6±13.5)与(25.2±11.0)秒],早产高蛋白组与足月高蛋白组比较[(29.6±13.5)与(23.8±11.6)秒],早产标准蛋白组与足月标准蛋白组比较[(25.2±11.0)与(23.5±10.4)秒],差异均无统计学意义(P﹥0.05)。比较海马CA1区m TOR阳性细胞数量,早产高蛋白组低于足月高蛋白组[(36.9±3.6)与(44.1±7.2)个],早产标准蛋白组低于足月标准蛋白组[(39.6±2.8)与(53.0±5.9)个](P值均﹤0.05);同胎龄中,不同蛋白组组间比较差异无统计学差异(P﹥0.05)。结论母鼠哺乳期高蛋白饮食干预,可促进早产大鼠的
Objective To explore the effects of high-protein supply for breast feeding rats on brain development of premature rats and its mechanism. Methods SPF class Sprague-Dawley rats were obtained; young rats of term group and premature group were all breastfed; maternal rats were given high- protein food or standard protein food during lactation. Young rats were divided into premature high protein group, premature standard protein group, term high protein group, term standard protein group,16 rats in each group. Rats of the experimental groups were given standard protein food from 22 days on end of lactation. Young rats were fed to 6 or 8 weeks, then Morris water maze of place navigation test was performed and escape latency was recorded. After place navigation test, the expression of mammalian target of rapamycin （mTOR） positive cells in the hippocampus of the brain was detected by immunohistochemistry. Statistical analysis was performed by variance analysis and t test. Results For the rats of 6 weeks, as training days extending, escape latency of rats of every group gradually shortened. On the fourth day, escape latency of premature standard protein group was higher than that of term standard protein group [ （40.5 ± 11.9） vs （28.3 ± 10.2） s ] ; the number ofmTOR positive cells ofhippocampal CAI area of premature standard protein group was lower than that of term standard protein group [ （35.2 ± 2.9） vs （45.7± 6.5） ] （both P 〈 0.05）. Although escape latency of premature high protein group was lower than that of premature standaxd protein group, but there was no significant difference [ （34.4 ± 12.8） vs （40.5 ± 11.9） s, P 〉 0.05 ] . Comparison of escape latency of premature high protein group and term high protein group [ （34.4±12.8） vs （28.1±16.4） s ] , the number of mTOR positive cells [ （36.1±3.7） vs （39.4±1.7） ] , there was no significant difference （both P 〉 0.05）. For the rats of 8 weeks, on the fourth day, comparison of escape latency between pr
作者
朱力逢
严彩满
王潜
苏志文
崔其亮
ZHU Li-feng;YAN Cai-man;WANG Qian;SU Zhi-wen;CUI Qi-liang(Department of Paediatrics, the Third A ftliated Hospital of Guangzhou Medical University, Guangdong, Guangzhou 510150, China;Department of Paediatrics, the First Hospital of Changsha, Hunan, Changsha 410005, China)
出处
《发育医学电子杂志》
2017年第4期233-237,252,共6页
Journal of Developmental Medicine (Electronic Version)
基金
广东省科技计划项目(粤科技规划字[2011]97号)
广州市科技计划项目(2014Y2-00167)
