摘要
降钙素原(Procalcitonin,PCT)是降钙素的前体物质,与人体细菌感染情况严重程度相关。通过PCT抗体与量子点偶联,自制试纸条,开发一种高灵敏度、快速便捷的PCT检测产品,对其初步应用。PCT单抗效价达107,PCT蛋白与量子点偶联,具有较好稳定性。试纸条线性检测范围0.15–120μg/L,灵敏度0.007μg/L,回收率范围91%–113%,批内批间变异系数小于8%。自制的荧光检测试纸条与市场上已有的降钙素原ELISA试剂盒进行比对,检测结果良好,基本能够完成临床样品的检测。
Procalcitonin（PCT） is the precursor of calcitonin related to the severity of human bacterial infection. We made a test strip by coupling anti-PCT to quantum dot, in order to develop a highly sensitive and convenient PCT testing product. The anti-PCT titer had reached 10~7 because of the stability by coupling anti-PCT with quantum dot. The detecting linear range of the experiment was 0.15 to 120 μg/L, the sensitivity was 0.007 μg/L, the recovery range was 91% to 113%, and the intra-and inter-assay coefficient of variation was less than 8%. Comparing the homemade fluorescence-detected test strip with PCT ELISA kit on sale, we got accurate results which could mostly accomplish the test of clinical samples.
作者
刘传志
李伟
刘桂莹
杨羽
宫平
侯玥
Chuanzhi Liu, Wei Li, Guiying Liu, Yu Yang, Ping Gong, and Yue Hou(College of Life Science and Technology, Changchun University of Science and Technology, Changchun 130021, Jilin, Chin)
出处
《生物工程学报》
CAS
CSCD
北大核心
2018年第3期440-448,共9页
Chinese Journal of Biotechnology
基金
吉林省教育厅"十三五"科学技术研究项目(吉教科合[2016]第385号)资助~~
关键词
降钙素原
荧光免疫层析
量子点
试纸条
procalcitonin, fluorescence immune chromatography, quantum dot, test strip
