摘要
目的通过分析体检人群人乳头瘤病毒(HPV)的感染情况,以了解云南省大理地区HPV的感染特点。方法选取2013年1月~2015年11月大理大学第一附属医院体检中心接诊的女性患者2912例,根据随机数字表法分为13型组(1587例)和21型组(1325例)。其中13型组应用荧光探针法进行13种高危型HPV检测,21型组应用分子导流杂交技术进行21型HPV亚型检测,并对两种检测技术的结果进行比较分析。结果 13型组中,检测出含HPV16、18、31、33、35、39、45、51、52、56、58、59、68等13种高危亚型的阳性患者206例,阳性率为12.98%(206/1587);21型组中,检测出阳性患者246例,阳性率为18.57%(246/1325);共检出除低危型HPV43型外的20种亚型;在20种HPV亚型感染中,HPV16型阳性率最高为29.67%(73/246),其次为HPV52亚型21.14%(52/246)。结论云南省大理地区HPV的感染分布具有异质性,两种检测方法的检出率无差异,检测方法的选择应该根据临床需要进行选择。
Objective To understand the characteristics of human papillomavirus （HPV） infection in Dali region of yun- nan province by analysis of the infection of HPV in the population of physical examination. Methods From January 2013 to November 2015, 2912 female patients received in Medical Examination Center of the First Affiliated Hospital of Dali University were selected, and divided into 13 type group （1587 cases） and 21 type group （1325 cases） according to the random number table. 13 type group was used to 13 types of high risk HPV detection by real-time PCR, 21 type group was used to type 21 HPV subtype detection by molecular hybridization, and the results of two kinds of detection techniques are compared and analyzed. Results In the 13 type group, a total of 206 cases of positive patients with 13 high-risk subtypes including HPV16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59 and 68 were detected,with a positive rate of 12.98%（206/1587）. In the 21 type group, 246 cases were infected with HPV, with the positive rate of 18.57% （246/ 1325）. A total of 20 subtypes except low-risk HPV43 were detected. The most common HPV genotypes were HPV16 with the positive rate of 29.67%（73/246）. Second, HPV52 subtype 21.14% （52/246） in 20 types of HPV subtypes. Con- clusion The infection of HPV in Dali region, Yunnan Province, has heterogeneity. The detection rate of the two detection methods was not different, and the selection of the detection method should be selected according to the clinical needs.
作者
赵立仙
毕磊
杨锡彤
王光明
何举
ZHAO Lixian;BI Lei;YANG Xitong;WANG Guangming;HE Ju(Department of Pathology, the First Affiliated Hospital of Dali University, Yunnan Province, Dali 671000, China)
出处
《中国医药导报》
CAS
2018年第7期131-134,共4页
China Medical Herald
关键词
人乳头瘤病毒
分子导流杂交
荧光探针法
宫颈癌
Human papillomavirus
Molecular hybridization
Real-time PCR
Cervical carcinoma
