摘要
本文利用两种不同的抗肿瘤药物阿霉素(DOX)和苯丁酸氮芥(Cb)混合,通过再沉淀法制备了一种新型的复合药物纳米粒子.1 H NMR证明了DOX和Cb混合后通过氨基和羧基静电力相互作用形成超分子复合物,通过DLS、SEM、AFM、TEM等表征证明了复合药物纳米粒子具有规整的形貌,较窄的尺寸分布.三种肿瘤细胞模型(MCF-7、A549、HepG2)的细胞毒性实验证明了复合药物纳米粒子相比于单独的DOX、Cb、DOX/Cb混合物有着更好杀死肿瘤细胞的效果.选取HepG2采用Hoechst染色检测凋亡细胞成像以及Western说明了复合药物纳米粒子能更好的诱导了肿瘤细胞的凋亡.共聚焦实验则很好的验证了相比于自由药阿霉素(DOX),复合药物纳米粒子可以更容易被肿瘤细胞内吞.
A novel composite drug nanoparticles （DOX-Cb nanoparticles） have been prepared by repre- cipitation after hydrophobic anticancer drug Doxorubicin （DOX） and hydrophobic anticancer drug chlor- ambucil （Cb） being mixed. H NMR confirms that DOX and Cb compound is formed via electrostatic force between amino group and carboxyl group. DLS, SEM, AFM and TEM confirm that Composite drug nanoparticles have regular morphology and a narrow size distribution. Cytotoxicity test by using three tumor models （MCF-7, A549, HepG2） shows Composite drug nanoparticles have better effect of killing tumor cells than free DOX, Cb and DOX/Cb mixture. Hoechst staining and westernresult by u- sing HepG2 suggest that Composite drug nanoparticles kill tumor by inducing tumor cells apoptosis. confocal experiment verifys DOX-Cb nanoparticles are easier endocytosed by tumor cells than DOX.
作者
徐越
吴传芳
XU Yue , WU Chuan-Fang(Departernent of Functional Genome, College of Life Sciences,Sichuan University,Chengdu 610065, Chin)
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2018年第2期394-400,共7页
Journal of Sichuan University(Natural Science Edition)
基金
国家自然科学基金(31300674)
