摘要
目的探讨猪Sus scrofa domesticus白细胞介素2(IL-2)和融合白细胞介素4/6(IL-4/6)基因的共表达对仔猪免疫应答的影响,为进一步研制新型免疫调节剂来加强仔猪抵御断奶后多系统衰竭综合征奠定基础。方法将猪IL-2和IL-4/6融合基因的共表达重组质粒,用壳聚糖材料包裹制成纳米颗粒,记作VRIL-4/6-2-CS。将仔猪分为2组,分别肌肉注射VRIL-4/6-2-CS(实验组)和生理盐水(对照组),2组均同时接种猪圆环病毒2型(PCV-2)疫苗,在接种后的第0、7、14、28天采集血样并分析免疫变化。结果实验组仔猪血清中的IgG2a抗体数量和血液中CD4^+、CD8+~T淋巴细胞数量均显著高于对照组(P<0.05);同时,实验组仔猪的IL-2、IL-4、IL-6、TNF-α、TLRs(TLR-2,7)、STATs(STAT-1,2,3)基因表达水平也显著高于对照组(P<0.05)。尽管2组之间PCV-2特异性抗体的含量差异无统计学意义,但是实验组仔猪的生长速率显著高于对照组(P<0.05)。结论 VRIL-4/6-2-CS能促进仔猪对PCV-2疫苗的免疫应答,是一种安全有效的免疫佐剂。
Objective In order to develop a novel effective immunomodulator to enhance pig resistance against post-weaning multisystemic wasting syndrome,we used the recombinant plasmid co-expressing the pig interleukin-2（IL-2） and fusion interleukin-4/6（IL-4/6） proteins which we constructed before,and studied its effect on immune response of piglets.Methods The recombinant plasmid was first encapsulated in chitosan nanoparticles by the ionotropic gelation method,and generated VRIL-4/6-2-CS.Then piglets were divided into 2 groups and intramuscularly injected with VRIL-4/6-2-CS or saline followed by the injection of pig circovirus-2（PCV-2） vaccine,respectively.The blood was collected from each piglet on days 0,7,14 and 28 to assay the immunological changes.Results The number of IgG2a,CD4~＋,CD8~＋T cells increased significantly in the sera or blood of piglets that treated with VRIL-4/6-2-CS（P 0.05）.Furthermore,the expression levels of IL-2,IL-4,IL-6,TNF-α,TLRs（TLR-2,7） and STATs（STAT-1,2,3） genes elevated significantly in the piglets that treated with VRIL-4/6-2-CS（P〈 0.05）.Although no significant differences were observed in the levels of PCV-2-specific antibody,the growth performance of VRIL-4/6-2-CS treated piglets was remarkably improved in comparison with that of control（P 〈0.05）.Conclusion VRIL-4/6-2 entrapped in chitosan is a promising effective adjuvant to promote the immune responses of pig vaccinated with PCV-2.
作者
陈祎
宋婷玉
李金海
肖永乐
曾光志
万小平
杨璐一
方鹏飞
王泽洲
高荣
CHEN Yi SONG Tingyu LI Jinhai2 , XIAO Yongle1 ZENG Guangzhi2 WAN Xiaoping1, YANG Luyi1 , FANG Pengfei2. , WANG Zezhou3. , GAO Rong1(1. Key Laboratory of Rio-Resource and Eeo-Environment of Ministry of Education, Key Laboratory for Animal Disease Prevention and Food Safety of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu 610065, China; 2. Sichuan Huapai Biopharmaeeutieal Company, Chengdu 610026, China; 3. Center for Animal Disease Control of Sichuan Province, Chengdu 610035, Chin)
出处
《四川动物》
北大核心
2018年第2期156-163,共8页
Sichuan Journal of Zoology
基金
国家自然科学基金项目(30871855)
科技部国际合作项目(2011DFA10101103)
