摘要
为了解白桦外生菌根真菌的群落结构,本研究选取了内蒙古赤峰市黑里河、赛罕乌拉以及阿拉善盟贺兰山3个国家自然保护区,采集白桦林下根围土壤,利用Illumina MiSeq测序平台对扩增子真菌ITS1区进行高通量测序。测序共获得外生菌根真菌序列1 041条,划分为404个OTU,隶属于2个门、6个纲、11个目、25个科、38个属,主要分布于担子菌门Basidiomycota。3个采样地共有优势外生菌根真菌OTU 21个,归属于红菇属Russula、乳菇属Lactarius、锁瑚菌属Clavulina、丝膜菌属Cortinarius、蜡壳耳属Sebacina、丝盖伞属Inocybe、Amphinema属、蜡伞属Hygrophorus,其中Russula占绝对优势。在黑里河与赛罕乌拉,Russula和Clavulina为绝对优势属;在贺兰山,Cortinarius和Sebacina为绝对优势属。土壤环境因子对群落中优势外生菌根真菌的影响程度依次为p H>Na>Ca>Mg>TN>K>TP>TC,其中pH、Ca与Mg对3个采样地的优势外生菌根真菌的分布差异造成显著影响。黑里河与赛罕乌拉的白桦外生菌根群落结构在属水平和优势属水平上相似性高于这两个采样地与贺兰山的白桦外生菌根群落结构。
The ectomycorrhizal（ECM）fungal community in the rhizospheric soil of Betula platyphylla in Inner Mongolia was investigated.Heilihe Natural Reserve,Saihanwula Natural Reserve and Helanshan Natural Reserve of Inner Mongolia were selected as sample plots.DNA ITS1 region of the fungi in rhizospheric soil were sequenced using the Illumina MiSeq High-throughput sequencing platform.A total of 1 041 sequences of ectomycorrhizal fungi obtained,dividing into 404 OTUs which belong to two phyla,six classes,eleven orders,25 families and 38 genera,being mainly distributed in Basidiomycota.The dominant ECM fungal OTUs totalled 21,belonging to Russula,Lactarius,Clavulina,Cortinarius,Sebacina,Inocybe,Amphinema and Hygrophorus,and Russula has the highest frequency.In Heilihe and Saihanwula,Russula and Clavulina are predominant,while Cortinarius and Sebacina are predominant in Helanshan.The degree of influence of soil environmental factors on ECM fungal descends in the order of pHNaCaMgTNKTPTC.The p H,Ca and Mg had significant effects on the distribution of dominant ECM fungi in these sample sites.Ectomycorrhizal fungal community of Betula platyphylla in Heilihe and Saihanwula is more similar than that in Helanshan at the genus level and dominant genus level.
作者
杨岳
闫伟
魏杰
YANG Yue YAN Wei WEI Jie(College of Forestry, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010019, Chin)
出处
《菌物学报》
CAS
CSCD
北大核心
2018年第3期294-304,共11页
Mycosystema
基金
国家自然科学基金(31360010)~~
关键词
外生菌根
高通量测序
可操作分类单元
优势属
ectomycorrhiza, high-throughput sequencing, operational taxonomic unit, dominant genera
