摘要
【目的】明确甲氰菊酯对苹果蠧蛾[Cydia pomonella(L.)]解毒酶系的影响。【方法】采用胃毒法测定甲氰菊酯对苹果蠹蛾3龄幼虫的LC_(10)和LC_(20)。以甲氰菊酯LC_(10)和LC_(20)质量浓度处理苹果蠧蛾3龄幼虫不同时间,测定其体内多功能氧化酶(MFOs)、羧酸酯酶(Car Es)和谷胱甘肽S-转移酶(GSTs)的活性变化。【结果】LC_(20)处理后,Car Es比活力在12、24、36、48 h时为对照的1.54、2.19、1.53和1.34倍,6 h和60 h时无明显变化;LC_(10)处理后,Car Es比活力在12、24、36 h时为对照的1.40、2.27和1.42倍。LC_(20)处理后GSTs比活力在12、24、36和48 h时均显著高于对照和LC_(10)处理(P<0.05);LC_(10)处理36 h和48 h时高于对照(P<0.05),其余时间均无明显变化。LC_(20)处理后,MFOs比活力在12、24和36 h时显著高于对照(P<0.05),48 h时显著低于对照(P<0.05),其余时间差异不显著;LC_(10)处理后12、24和36 h显著高于对照(P<0.05),其余时间差异均不显著。Car Es的K_m在LC_(20)处理后6、12、24、36、48和60 h分别比对照降低了40.00%、37.23%、32.17%、29.51%、52.94%和28.09%,V_(max)在6、12和48 h分别比对照升高了24.59%、19.36%和81.97%,差异显著(P<0.05);GSTs的K_m值和V_(max)变化趋势与Car Es相反;MFOs的K_m值在48 h显著低于对照(P<0.05),其他时间点无明显变化,V_(max)在12和48 h时无明显变化,其余时间点均显著低于对照(P<0.05)。【结论】LC_(10)和LC_(20)甲氰菊酯处理后的苹果蠹蛾体内Car Es、MFOs和GSTs均有解毒作用,但Car Es起主导作用。
【Objective】The use of insecticides to control coding moths Cydia pomonella in the apple orchards in China is essential and as a result,the development of insecticide resistance in pest populations is of major concern.C.pomonella is one of the main quarantine pests of fruits in apple production and insecticide resistance of this species is little known in a number of countries.The aim of this study is to investigate various detoxification enzymes including multifunctional oxidase（MFO）,carboxylesterase（Car E）and glutathione s-transferase（GST）that are involved in C.pomonella resistance to fenpropathrin by testing the effects of fenpropathrin on the activities of detoxifying enzymes in the larvae of C.pomonella,and by also observing the impact of the sublethal effects on the survival of individual insects after using fenpropathrin which can result in the change in ecological behavior,reproduction and development and so on.【Methods】The coding moths were originally collected from abandoned apple orchards in Wuwei city,Gansu province and maintained under standard laboratory conditions of（25±1）℃,a relative humidity of（75±5）%,with a 16∶8 light∶dark cycle;after which a third of the instar larvae of the coding moths were chosen to be the tested insects.First,prepare the five concentration gradient 2.5,5,10,20 and 40 mg·L^（-1）by using raw fenpropathrin and 60%acetone as the solvent,spraying it into the feed block containing 24-holes in which the tested insects were put after being starved for four hours.The LC_（50）virulence regression equation was established according to the death status of tested insects after being treated for 72 h by using the method of stomach toxicity,of which,different logarithm concentrations of fenpropathrin are set as the abscissa and the mortality probability value of the third instar of C.pomonella is set as the ordinate.Then the sublethal doses of LC_（10）and LC_（20）of fenpropathrin on the third instar larvae of C.pomonella were calculated acco
作者
尚素琴
柳永花
刘宁
张彩虹
他光崇
SHANG Suqin, LIU Yonghua, LIU Ning, ZHANG Caihong, TA Guangchong(College of Plant Protection of Gansu Agricultural University. Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Prov- ince, Lanzhou 730070, Gansu, Chin)
出处
《果树学报》
CAS
CSCD
北大核心
2018年第3期326-333,共8页
Journal of Fruit Science
基金
甘肃省省级大学生创新创业训练计划(201610733002)
关键词
苹果蠹蛾
甲氰菊酯
解毒酶
亚致死效应
抗药性
Cydia pomonella
Fenpropathrin
Detoxifying enzymes
Sublethal concentration
Resistance
