摘要
抗菌肽具有广谱抗菌特性,有望成为抗生素较好的替代产品.研究抗菌肽的抗菌机制,可以为新型抗菌肽的设计提供指导.无论抗菌肽采用哪种抗菌机制,其首先要稳定地吸附到细胞膜之上.因此,本文利用分子动力学模拟方法比较了抗菌小肽BLFcin6与5种不同细胞膜之间的相互作用.对这5种细胞膜而言,小肽会很快结合在POPG膜和DPPC-CHOL膜的表面,倾向于进入DPPC膜的疏水内部,与POPC膜和POPC-CHOL膜的接触很少.考察相互作用能,小肽与POPG膜的相互作用最强,主要是小肽与细胞膜亲水头部存在静电相互作用;小肽与DPPC膜的疏水尾部的相互作用较强,但受胆固醇影响,小肽只结合在DPPC-CHOL膜表面.在结合过程中,小肽N端的Arg会先结合到细胞膜上,静电相互作用在小肽锚定细胞膜的过程中起关键作用.以上研究从原子水平上解释了为什么BLFcin6小肽具有抗菌作用,哪些残基起关键作用,也为进一步开展BLFcin6小肽及其衍生小肽的研究奠定基础.
Antimicrobial peptides have broad spectrum of antibacterial properties and are expected to become the better altematives of antibiotics. Studies on the antibacterial mechanism can provide guidance for the design of new antibacterial peptides. No matter what kind of antibacterial mechanism, antibacterial peptides adsorb on the cell membrane firstly. In this manuscript, the molecular dynamic simulations were used to study the interaction between antimicrobial peptide BLFcin6 and five different membranes. For five kinds of cell membranes, the peptide combined with the surface of DPPC-CHOL membrane and POPG membrane rapidly, and tend to entered the hydrophobic interior of DPPC membrane. However, the peptide have little contact with POPC-CHOL membrane and POPC membrane. In terms of interaction energy, the peptide and POPG membrane have the strongest interaction, which mainly arise through the electrostatic interaction between the pepetide and the hydrophilic head of POPG membrane. For peptide and DPPC membrane, the interaction are mainly arise between the peptide and the hydrophobic tail of DPPC membrane. However, the peptide only combined with the surface of DPPC-CHOL membrane because of the effects of cholesterol. In the process of combination, the N-terminal Argnine residues contact with the cell membrane firstly, electrostatic interaction plays a key role in the process of peptide anchor in the cell membrane. The research explain why BLFcin6 peptide have antibacterial effect at the atomic level, which are the key residues, and also provide help for the further study of BLFcin6 peptide and its derivatives.
作者
孔真真
赵立岭
唐艳
王吉华
曹赞霞
KONG Zhen-Zhen1,4）, ZHAO Li-Ling1,2）, TANG Yan1,3）, WANG Ji-Hua1,2）, CAO Zan-Xia1,2）＊(1） Shandong Provincial Key Laboratory of Biological Physics, Dezhou University, Dezhou 253023, China; 2） School of Physics and Electronic Information, Dezhou University, Dezhou 253023, China; 3） School of Resources Environment and Planning, Dezhou University, Dezhou 253023, China; 4） School of Life Science, Shandong Normal University, Jinan 250014, Chin)
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2018年第3期351-362,共12页
Progress In Biochemistry and Biophysics
基金
国家自然科学基金(31670727
61671107)
山东省自然科学基金(ZR2016CQ15
ZR2014AL014)
山东省科学发展计划(2014GNC110025)资助项目~~
关键词
BLFcin6抗菌小肽
不同成分磷脂膜
分子动力学模拟
肽膜相互作用
BLFcin6 antimicrobial peptide, different compositions of the phospholipid membrane, moleculardynamics simulation, peptide-membranes interaction
