摘要
目的分析菏泽市手足口病病原体检出情况及流行病学特征,为手足口病防控提供可靠的实验室依据。方法采用实时荧光定量PCR,对2010~2016年菏泽市收集的临床确诊病例的粪便标本,提取核酸,进行肠道病毒71型(EV71)、柯萨奇病毒A16型(CoxA16)和其他肠道病毒核酸检测。结果 2010~2016年共收集样本3 231份,其中阳性2 881份,阳性率为89.17%。EV71阳性1 520份,阳性率为47.04%;CoxA16阳性576份,阳性率为17.83%;其他肠道病毒阳性756份,阳性率为23.40%(P<0.05)。重症病例以EV71为主344份,占81.52%(344/422)(P<0.01);发病年龄主要集中在≤5岁儿童,以1~3岁构成比较高(70.32%)(P<0.01);男性发病(65.88%)高于女性(34.12%)(P>0.05);发病高峰集中在3~5月(57.34%)(P>0.05)。结论菏泽市手足口病发病趋势已从2010年的EV71和CoxA16共同主导流行,转变为EV71与CoxA16交替流行。
Objective To analyze the pathogenic detection and epidemiological characteristics of hand-foot mouth disease in Heze city,2010-2016 ,so as to provide the basis for the prevention and control. Methods Samples were collected from designated medical institutions of HFMD in Heze city during 2010-2016 ,real-time fluorescent quantitative PCR was used to detect the nucleic acid of EV71,CoxA16 and other EV. Results During 2010-2016,a total of 3 231 cases were detec ted, and 2 881 cases were positive, the positive rate was 89.17 %. 1 520 were EV71 positive, the positive rate was 47.04 %, 576 were CoxA16 positive, the positive rate was 17.83 %,756 were other EV positive, the positive rate was 23.40 0/0 （ 〈 0.05）.The severe HFMD was mainly caused by EV71,with 344 samples,accounting for 81.52%（344/422）（P〈 0.01）. HFMD cases were mainly concentrated in children under 5 years old, especially 1-3 years old（70. 32%） （ P 〈0.01） ,and that of the males （65.88 %）was higher than that of the females（34.12%）（ P 〉0.05）. The epidemic peak was from March to May（57.34%）（ P 〉0.05）. Conclusion The main pathogens of HFMD have been changed from EV71 and CoxA16 to EV71 alternating CoxA16 in Heze city.
出处
《预防医学论坛》
2017年第12期930-933,共4页
Preventive Medicine Tribune
