摘要
目的探讨铝暴露工人精子线粒体早老素相关菱形蛋白(PARL)水平与精子活力、存活率及畸形率的相关性。方法从广西某大型铝企业选取162名男性铝作业工人作为铝暴露组,同时选取年龄、工龄构成匹配的162名企业服务公司员工为对照组;分别采用石墨炉原子吸收光谱法、高效液相色谱法测定作业环境铝浓度,工人血液和尿液铝浓度;利用酶联免疫吸附试验测定精子线粒体PARL蛋白水平,并对精子功能指标进行分析。结果铝暴露组配料、电解和铸造3个车间空间平均铝浓度分别为(6.72±1.45)、(7.23±1.50)、(7.35±1.72)mg/m3,明显高于对照组环境中铝浓度(F=8.314,P<0.001)。铝暴露组血液和尿液铝浓度均明显高于对照组(P<0.05),而精子线粒体PARL蛋白水平则明显低于对照组(P<0.05)。铝暴露组精子存活率和精子活力均明显低于对照组,精子畸形率显著高于对照组(P<0.05)。精子活力、精子存活率与精子线粒体PARL蛋白水平呈正相关(r精子活力=0.713,P=0.012;r精子存活率=0.628,P=0.008);精子畸形率与精子线粒体PARL蛋白水平呈负相关(r精子畸形率=-0.953,P=0.002)。结论铝暴露作业工人精子功能显著受损,且精子活力、存活率及畸形率与PARL蛋白水平密切相关。
Objective To investigate the correlation between the level of presenilin-associated rhomboidlike protein（PARL）and the sperm motility,survival rate and deformity rate of sperm in workers exposed to aluminum.Methods A total of 162 male workers exposed to aluminum in a large aluminum enterprise in Guangxi were selected as the exposure group,and the 162 staff members of the service company were selected as the control group by matching the age and length of service.The concentration of aluminum in the working environment and the aluminum content in the blood and urine were determined by graphite furnace atomic absorption spectrometry and high performance liquid chromatography.Enzyme linked immunosorbent assay was used to measure the level of PARL protein in sperm and sperm function was evaluated.Results The average concentrations of aluminum in the batching,electrolysis and casting of aluminum exposure group were（6.72±1.45）,（7.23±1.50）and（7.35±1.72）mg/m^3,which were significantly higher than those of the control group（F=8.314,P〈0.001）.The content of aluminum in blood and urine of exposed group were significantly higher than those of control group（P〈0.05）,while the levels of PARL of exposed group were significantly lower than those of control group（P〈0.05）.Moreover,the sperm survival rate and sperm motility of exposed group were significantly lower than those of the control group,and the sperm deformity rates of exposed group were significantly higher than those of the control group（P〈0.05）.Pearson correlation analysis showed that there was a positive correlation of sperm motility,sperm survival rate and protein level of PARL（r_（sperm motility）=0.713,P=0.012;rsperm survival rate=0.628,P=0.008）;while the sperm deformity rate and protein level of PARL showed a significant negative correlation（r_（sperm deformity rate）=0.953,P=0.002）.Conclusion The sperm function was significantly impaired in aluminum exposed workers,and the changes of sperm motility,survival rate
出处
《国际检验医学杂志》
CAS
2018年第5期608-611,共4页
International Journal of Laboratory Medicine
基金
百色市科学研究与技术开发计划资助项目(百科计20161507)
