摘要
目的探讨叉头框C2(FOXC-2)、YB-1及相关蛋白在胃癌发生发展及侵袭转移中的作用。方法选取193例胃组织标本,包括正常胃黏膜50例,胃黏膜上皮内瘤变50例,胃癌淋巴结未转移组织19例,原发灶组织93例,胃癌患者淋巴结转移组织74例,淋巴结未转移组织19例,原发灶远处转移组织33例作为研究对象。采用免疫组织化学法检测各类组织中FOXC-2、YB-1、E-钙黏蛋白(E-cadherin)、波形蛋白(Vimentin)、基质金属蛋白酶-2(MMP-2)的表达及分布情况。结果同正常及上皮内瘤变胃黏膜相比,胃癌原发灶组织中FOXC-2,YB-1,Vimentin和MMP-2表达升高,E-cadherin的表达降低,差异具有统计学意义(P<0.05)。FOXC-2、YB-1的表达与E-cadherin的低表达和Vimentin的高表达及MMP-2的表达之间存在弱相关。随着胃癌临床分期提高,淋巴结转移和远处转移出现,FOXC-2蛋白的表达增强(P<0.05)。随着胃癌临床分期的增高,分化程度的降低,浸润深度的加深及淋巴结转移,远处转移的出现,YB-1蛋白的阳性表达亦增高(P<0.05)。胃癌分化程度降低,浸润深度加深,淋巴结转移和远处转移出现MMP-2蛋白的表达也随之增强(P<0.05)。结论 FOXC-2、YB-1与胃癌的发生发展及侵袭转移有关,可能机制是通过激活上皮间质转化过程及上调转移相关分子MMP-2的表达从而促进癌细胞的侵袭转移。
Objective To investigate the expression of FOXC-2, YB-1 and related proteins and their influences on development, invasion and metastases in gastric carcinoma. Methods A total of 193 tissue samples were collected, including 50 cases of normal gastric mucosa,50 cases of gastric mucosal intraepithelial neoplasia and 93 cases of primary gastric carcinoma. The 93 cases of primary gastric carcinoma included 74 cases of positive lymph node metastasis tissues, 19 cases of nonmetastasis tissues and 33 cases of distant metastasis tissues. Immuohistochemistry was used to detect the expression and distribution of FOXC-2, YB-1, E-cadherin,Vimentin and MMP-2 in normal and intraepithelial neoplasia, gastric carcinoma, positive lymph node metastasis tissues and distant metastasis tissues. Results The expressions of FOXC-2, YB-1, Vimentin and MMP-2 in gastric carcinoma were significantly higher than those in normal and intraepithelial neoplasia while the expression of E-cadherin was sigaificantly lower （P〈0.05）. The expressions of FOXC-2, YB-1 were significantly correlated with low expression of E-cadherin and high expression of Vimentin and MMP-2 （P〈 0. 05）. The expression of FOXC-2 protein was significantly correlated with TNM stage, lymph node metastasis and distant metastases （P〈0.05）. The expression of YB-1 protein was significantly correlated with TNM stage, differentiation degree, invasion depth, lymph node metastasis and distant metastasis （P〈0.05）. The expression of MMP-2 protein was closely related to the degree of differentiation, invasion depth, lymph node metastasis and distant metastasis （P〈0. 05）. Conclusion FOXC-2, YB-1 may be related to the occurrence, development, invasion and metastasis of gastric carcinoma. The possible mechanism is to promote the invasion and metastasis of cancer ceils by activating the epithelial mesenchymal transition process and up regulating the expression of MMP-2.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2018年第2期215-220,共6页
Journal of Sichuan University(Medical Sciences)
基金
国家自然科学基金(No.81173386)
安徽省高等学校自然科学研究一般项目(No.KJ2015B045by
No.KJ2015B040by)资助
