摘要
甲基腐胺氧化酶(MPO)是茄科植物中腐胺衍生的生物碱合成途径中重要基因。前人研究表明,MPO基因由DAO基因进化而来,然而其在植物中的完整进化图景未见报道。本研究在全基因组水平上全面鉴定MPO基因所属的CuAO基因的基础上,通过进化分析,构建了植物的MPO基因进化路径。首先,从已报道的MPO、CuAO及DAO基因中均鉴定得到了三个保守结构域;在此基础上,结合BLAST和HMMER,从6个植物基因组中鉴定得到了35个CuAO家族基因。系统发育分析表明,该家族基因分为4个亚家族。亚家族Ⅰ和Ⅱ均为AO基因;亚家族Ⅲ基因功能还未获鉴定,该亚家族基因在烟草属植物中不存在;亚家族Ⅳ基因仅在菊亚纲中发现,其在茄科中进一步分化成DAO基因和MPO基因。本研究表明,MPO基因的起源最早可追溯到AO基因,而其最近的祖先出现于菊亚纲,最终在茄科植物起源后进化得到具有MPO活性的基因,从而整合入尼古丁等腐胺衍生的生物碱生物合成途径。
Methylputrescine oxidase（MPO） is an important gene in biosynthesis pathway of putrescine-derivative alkaloids in Solanaceae.Previous studies indicated that MPO evolved from DAO,yet its whole evolutionary path in plant has not been reported.On the basis of genome-wide identification,this study completely identified CuAO gene family,to which MPO belonged.Then,the evolutionary path of MPO gene in plants was constructed by evolutionary analysis.Firstly,three conserved domains were identified in the three known MPO,CuAO and DAO genes.On this basis,35 CuAO family genes were identified from 6 plant genomes through the combination of BLAST and HMMER.Phylogenetic analysis showed that the family could be divided into 4 subfamilies.Both subfamiliesⅠand Ⅱ were AO genes.Genes in subfamily Ⅲ had not been functionally characterized,and the genes in this subfamily did not exist in Nicotiana plant.Genes of subfamily Ⅳ were only distributed in Asterids,and it was split into DAO and MPO in Solanaceae.Our results indicated that MPO genes could be traced back to AO genes in plants,whose nearest ancestor genes appeared in Asterids.Finally,after the origin of Solanaceae,the gene with MPO activity was evolved.Then,it was integrated into the alkaloid biosynthesis pathway of putrescinederivative such as nicotine.
出处
《分子植物育种》
CAS
CSCD
北大核心
2018年第1期47-53,共7页
Molecular Plant Breeding
基金
中央级科研单位基本科研业务费(1630032014013
1630032013009)资助
关键词
腐胺甲基氧化酶
含铜氨基氧化酶
基因挖掘
进化
Methylputrescine oxidase
Copper-containing amine oxidase
Gene mining
Evolution
