摘要
以不同浓度的氯化钾(KCl)和γ-氨基丁酸(GABA)处理皱纹盘鲍后期面盘幼虫,处理不同时间后,观察并分析KCl和GABA对皱纹盘鲍幼虫附着变态的诱导作用。结果表明,低浓度组,KCl(10mmol/L、12h)和GABA(10-6 mmol/L、6和12h)均可显著提高其附着和变态率,GABA组诱导效果优于KCl组,GABA(10^(-6) mmol/L、6h)处理组优于12h处理组。高浓度组,KCl(100、200和300mmol/L)和GABA(10^(-4)、10^(-3)和10^(-2) mmol/L)随着浓度的提升和诱导时间的延长,幼虫附着变态率降低,死亡率升高。综合考虑附着率、变态率、死亡率及实验成本等因素,低浓度的KCl(10 mmol/L、12h)和GABA(10^(-6) mmol/L、6h)都可以广泛应用于生产中,来提高幼虫的附着变态率,增加发育的同步性,降低死亡率。
The impact of potassium chloride (KC1) and )γ-aminobutyric acid (GABA) solutions on settlement and metamorphosis of the post veliger of Haliotis discus hannaiwas analyzed in the present work. The results showed that the settlement rates were significantly increased inKCl(10 mmol/L, 12h)expo sure and GABA(10^-6mmol/L, 6 and 12h) exposure,and the exposure of GABA was better than KCl. The settlement and metamorphosis rates in GABA treated with 6 hours were better than that treated with 12 hours. The higher concentrations of KCl (100, 200, 300 mmol/L) and GABA (10^-4 , 10^- 3 and 10^-2mmol/L) could also induce the metamorphosis, the mortality rate might reduce with increasing KCl and GABA concentration and induction time. Therefore, the combination of KC1 (10 mmol/L, 12h) and GABA (10^-6mmol/L,6h) treatment for the post veliger was recommended to rise the settlement and metamorphosis rates, to increase the synchronism development and to reducethe mortality rate. This method can he used widely in the production.
出处
《海洋湖沼通报》
CSCD
北大核心
2018年第1期123-128,共6页
Transactions of Oceanology and Limnology
基金
山东省农业良种工程项目(鲁农良种【2014】1号)经费资助