摘要
目的通过制备柚皮素-β-环糊精(β-CD)包合物,改善柚皮素的溶解度,研究柚皮素对对乙酰氨基酚(APAP)诱导的小鼠急性肝损伤(ALI)的保护作用。方法将60只雄性健康昆明种小鼠随机分为正常对照组,柚皮素-β-CD包合物低剂量组(12.5 mg/kg)、柚皮素-β-CD包合物高剂量组(50mg/kg)和模型组,正常对照组和模型组给予生理盐水,连续给药7 d,末次给药12 h后,除正常对照组外,其余各组均腹腔注射APAP制备小鼠ALI模型,腹腔注射后禁食不禁水24 h,测定各组小鼠存活率,摘眼球取血,检测血清中谷丙转氨酶(ALT)和谷草转氨酶(AST)的活性,腹腔解剖取肝脏计算肝指数,比色法检测肝组织SOD、MDA含量和GSH-PX活性,HE染色检测肝组织病理变化。结果β-CD使柚皮素的溶解度提高了5.56倍。与正常对照组相比,模型组的死亡率、肝指数、血清ALT、AST活性均升高(P均<0.05)。与模型组比较,柚皮素-β-CD包合物各组的死亡率、肝指数、血清ALT、AST活性以及MDA的含量均明显降低(P均<0.01),而SOD和GSH-PX活性明显升高(P均<0.05)。HE染色结果显示,模型组肝组织结构破坏,许多肝细胞空泡变性坏死,中央静脉淤血严重,而柚皮素-β-CD包合物各组肝小叶结构清晰,有少量肝细胞变性,未见明显的肝淤血。结论柚皮素对APAP所致的ALI具有保护性作用,其机制可能与柚皮素的抗氧化作用有关。
Objective To prepare the inclusion complex of naringenin-β-cyclodextrin（ β-CD） and to investigate its protective effect and mechanism in acute liver injury induced by acetaminophen in mice. Methods 60 male KM mice were randomly divided into 4 groups（ n = 15）,including normal control group,naringenin low-dose group（ 12. 5 mg/kg）,naringenin high-dose group（ 50 mg/kg） and acetaminophen model group. All groups were given medicines or normal saline via intraperitoneal injection,once a daily for 7 consecutive days.12 h after the last administration,all animals were given acetaminophen with the dose of 500 mg/kg except normal control group. After 24 h,the survival rate of mice was determined,blood samples were collected,and the activities of alanine transaminase（ ALT） and aspartate transaminase（ AST） were evaluated. The hepatic index,the activities of superoxide dismutase（ SOD）,glutathione peroxidase（ GSH-PX） and the content of Malondialdehyde（ MDA） in liver tissue were measured. HE staining was used to detect the structure changes. Results β-cyclodextrin increased solubility of naringenin up to 5. 56 times. Compared with control group,the mortality,the hepatic index,ALT and AST levels were increased in model group（ P 0. 05）. Compared with model group,the mortality,the hepatic index,the activities of ALT and AST and the content of MDA in naringenin group were decreased considerably（ P 0. 01）,while the activities of SOD and GSH-PX were obviously elevated（ P 0. 05）.HE staining showedclear hepatic lobules in each group of naringenin,with few hepatic cell degeneration and congestion. Conclusion Naringenin has protective effects on acetaminophen-induced acute liver injury,and the mechanism may be related to its antioxidant activity.
出处
《湖北科技学院学报（医学版）》
2018年第1期1-4,共4页
Journal of Hubei University of Science and Technology(Medical Sciences)
基金
国家自然科学基金青年科学基金项目(81502635)
湖北省教育厅科学研究计划资助项目(D20152803)
地方高校国家级大学生创新创业训练计划项目(201710927012)
