摘要
为进一步开发和利用辣木叶资源,筛选用于降血糖的活性成分,采用响应面分析法优化微波辅助提取辣木叶总黄酮的工艺,应用AB-8型大孔树脂层析柱和聚酰胺层析柱联用的工艺对辣木叶总黄酮进行纯化,并分析纯化前后辣木叶总黄酮对α-葡萄糖苷酶的抑制效果及抑制作用类型。结果表明,辣木叶总黄酮最佳提取条件为:时间308 s、功率302 W、乙醇浓度75%、料液比1∶52(m/v),在此条件下总黄酮提取率为5.53%±0.11%;纯化后辣木叶总黄酮的纯度为764.49±25.17 mg·g^(-1)。辣木叶总黄酮对α-葡萄糖苷酶具有抑制作用,纯化后的辣木叶总黄酮的抑制效果增强,其半数抑制浓度(IC50)为4.18 mg·m L^(-1),抑制作用类型为典型的竞争性抑制。本研究结果为辣木叶中黄酮类化合物的开发利用提供了技术参考,也为研发预防和治疗降血糖的药物或功能性食品提供了新的依据。
In order to further utilize Moringa oleifera Lam. leaves resource and screen for active ingredients for hypoglycemia treatment,response surface analysis methodology was applied to optimize the microwave-assisted extraction process of total flavonoids from Moringa oleifera Lam. leaves（ TFM）. AB-8 macroporous resin and polyamide resin chromatography column were used to purify the TFM. The inhibitory effect and inhibition type of TFM against α-glucosidase were measured. Results showed that the optimum extraction conditions were 308 s,power of 302 W,ethanol concentration of 75%,ratio of material to liquid as 1 ∶ 52（ m/v）. Under the optimum extraction condition,the total flavonoids yield was 5. 53% ± 0. 11%. After purification,the purity of the TFM can reach 764. 49 ± 25. 17 mg·g-1.TFM had an inhibitory effect on α-glucosidase,and the inhibitory effect was enhanced after purification. The IC50（ half maximal inhibitory concentration） of purified TFM against α-glucosidase was 4. 18 mg·m L^-1,and the inhibition type was typical competitive inhibition. The results of this study provide technical references for the development and utilization of flavonoids from Moringa oleifera Lam. leaves,and also contributes to the development of drugs or functional foods for hypoglycemia treatment.
出处
《核农学报》
CAS
CSCD
北大核心
2018年第1期84-94,共11页
Journal of Nuclear Agricultural Sciences
基金
浙江省自然科学基金项目(LY12C19011)
食品科学与工程浙江省重中之重一级学科基金项目(2017SIAR219)
关键词
辣木叶
总黄酮
微波辅助提取
响应面
Α-葡萄糖苷酶
Moringa oleifera Lam. leaves, total flavonoids, microwave assisted extraction, response surface, α -glucosidase
