摘要
为明确eIF5B-1基因在拟南芥种子萌发过程中的生理作用。以拟南芥翻译起始因子的T-DNA插入突变体eif5b-1为材料。测定突变体eif5b-1种子的萌发速率,同时利用多聚核糖体谱和qRT-PCR的研究方法,对拟南芥野生型(WT)和eif5b-1突变体的萌发过程进行了研究。结果表明:1)eif5b-1突变体种子的萌发率下降;2)eif5b-1突变体种子萌发过程中多聚核糖体的形成受到影响;3)eif5b-1突变体中ABI1、ABI3、ABI4、ABI5及DOG1基因的转录受到影响;4)eif5b-1突变体选择性地影响ABI4和DOG1基因的翻译效率。
A novel T-DNA insertion mutant of Arabidopsis translation initiation factor,eif5 b-1,was identified to study the regulation of seed germination in Arabidopsis.Compared to WT,the germination rate of eif5 b-1 mutant seeds was investigated.Using polysome profiling and qRT-PCR,the germination process of WT and eif5 b-1 mutant were analyzed.The results showed that：1）The germination rate of eif5 b-1 mutant was decreased;2）The polysome profiling was abnormal in eif5 b-1 mutant;3）The transcription levels of ABI1,ABI3,ABI4,ABI5 and DOG1 were changed in eif5 b-1 mutant;4）The translation efficiency of ABI4 and DOG1 were selectively changed in eif5 b-1 mutant.
出处
《中国农业大学学报》
CAS
CSCD
北大核心
2018年第1期12-18,共7页
Journal of China Agricultural University
基金
国家自然科学基金项目(31571747)
转基因重大专项(2016ZX08002-002)
关键词
拟南芥
翻译起始因子
种子
萌发
翻译
脱落酸
Arabidopsis
translation initiation factor
seeds
germination
translation
ABA
