摘要
目的:探讨龙琥醒脑颗粒对脑出血(ICH)模型小鼠cAMP/PKA/AQP4信号通路的调控作用。方法:参照Rynkowski法建立小鼠ICH模型,将60只雄性ICH小鼠随机分为正常组、假手术组、模型组、龙琥醒脑颗粒高剂量组和龙琥醒脑颗粒低剂量组,每组12只,造模成功后,中药高、低剂量组小鼠分别灌胃给予20 g/kg、10 g/kg的龙琥醒脑颗粒溶液,正常对照组、假手术组和模型组小鼠则给予等量蒸馏水。采用Longa法,对各组小鼠分别于给药7 d、14 d后进行神经功能缺损评分;采用干湿重法评估ICH后血肿周围脑组织含水量;分别采用荧光定量PCR(qPCR)法和western blotting法检测末次给药后各组小鼠脑组织中cAMP、PKA和AQP4基因和蛋白表达。结果:与模型组比较,龙琥醒脑颗粒高、低剂量组均能显著降低神经功能缺损评分和脑组织含水量(P<0.05),同时明显下调cAMP、PKA和AQP4基因和蛋白表达水平(P<0.05)。结论:龙琥醒脑颗粒对ICH模型小鼠cAMP/PKA/AQP4信号通路具有明显的调控作用,这可能是其抗神经功能缺损、减轻脑水肿而治疗ICH的重要机制之一。
Objective： To investigate the effect of Longhu Xingnao granule （LXG） on intracerebral hemorrhage in rats and the regulation of cAMP/PKA/AQP4 signaling pathway. Methods： A mouse ICH model was estab- lished by Rynkowski method. Sixty male ICH mice were randomly divided into normal group, sham operation group, model group, LXG high-dose （20 g/kg） group and low-dose （10 g/kg） group. Mice in high-dose and low-dose groups were given LXG via gavage for two weeks after modeling, and mice ih other groups were giv- en equal volume of saline. Neurological deficits was assessed by Longa＇ method on day 7 and day 14 after ad- ministration. Brain edema was quantitated by dry/wet weight. The mRNA and protein expressions of cAMP, PKA and AQP4 in brain tissues were detected by qPCR and western blotting, respectively. Results： Neurologi- cal deficits grade and brain water content were markedly increased in the model group when compared with the normal group and sham operation group （P〈 0. 05）. Compared with model group, neurological deficits grade and brain tissue water content were obviously decreased in LXG high-dose group and low-dose group （P〈0. 05）. The mRNA and protein expressions of cAMP, PKA and AQP4 in brain tissues were notably down-regulated in LXG high-dose group and low-dose group （P〈0. 05）. Conclusion： LXG attenuated neurological impairment and encephaledema in ICH mice probably via the regulation of the cAMP/PKA/AQP4 signaling pathway.
出处
《广西医科大学学报》
CAS
2018年第2期162-165,共4页
Journal of Guangxi Medical University
基金
长沙市科技计划项目(No.KQ1606006)
