摘要
目的研究不同浓度虫草素对人骨肉瘤细胞株MG63增殖与凋亡的影响。方法不同浓度虫草素作用于MG63细胞48 h后,倒置显微镜观察细胞形态变化,MTS法检测虫草素对MG63细胞增殖活性的影响,流式细胞术检测虫草素对MG63细胞周期和凋亡的作用,Hoechst 33342染色和Western blot检测虫草素对骨肉瘤细胞凋亡的影响。结果虫草素对人骨肉瘤细胞增殖有显著抑制作用,且呈浓度和时间依赖性。流式细胞术结果显示该细胞被阻滞在S期和G_2/M期(P<0.05),同时虫草素可引起MG63细胞早期凋亡(P<0.05)。Western blot结果显示,随虫草素浓度增加,Bcl-2蛋白表达下调,而Bax蛋白表达上调,且各实验组均出现蛋白分子量为89 k D的PARP裂解条带。结论虫草素通过下调MG63细胞中凋亡抑制蛋白Bcl-2并上调促凋亡蛋白Bax表达,同时阻滞细胞周期进展,促进早期凋亡。
Objective To study the effect of different concentrations of cordycepin on the proliferation and apop- tosis of human osteosarcoma cell line MG63. Methods Different concentrations of cordyceps were given to MG63 cells and sustained for 48 h. The morphological changes were observed by microscope. The effect of eordyeepin on the prolifera- tion of MG63 cells was detected by MTS. The effect of eordycepin on the cell cycle and apoptosis of MG63 cells was deter- mined by flow eytometry. The effects of cordycepin on apoptosis of osteosarcoma cells were examined by Hoechst 33342 staining and Western blot. Results Cordyeepin had a significant inhibitory effect on the proliferation of human osteosar- coma cells in concentration - and time - dependent manners. The results of flow eytometry showed that the cells were blocked in S and G2/M phase （ P 〈 0. 05 ）, and cordyceps could induce early apoptosis of MG63 cells （ P 〈 0. 05 ）. The results of Western blot showed that the expression level of Bel - 2 protein was down - regulated ; Bax was up - regulated a- long with the increase of the concentration of cordycepin. The cleaved - PARP with molecular weight of 89 kD was deter- mined in each experimental group. Conclusion Cordycepin can down - regulate the expression of Bcl - 2 and up - regu- late the pro- apoptotic protein Bax in MG63 cells, block the cell cycle, and promote early apoptosis.
出处
《广东医学》
CAS
2018年第4期506-510,共5页
Guangdong Medical Journal
基金
2017年度广东大学生科技创新培育专项资金项目("攀登计划"专项资金)(编号:pdjh2017b0220)
广东医科大学大学生创新实验项目立项课题(编号:2016FZZY001)
