摘要
目的研究miR-207在大鼠肾脏纤维化(RF)模型中的差异表达,并对其功能进行研究。方法实验组大鼠(UUO)分离并结扎右侧输尿管,假手术组只分离右侧输尿管,不结扎。Real-time PCR定量分析大鼠肾脏纤维化模型肾脏和尿液中miR-207的表达,以及瞬时转染miR-207 mimics和inhibitors的大鼠肾小管上皮(NRK-52E)细胞中纤维化相关基因的表达。结果大鼠肾脏纤维化模型尿液和肾脏组织中miR-207表达上调(P<0.05),miR-207能上调大鼠肾小管上皮NRK-52E细胞中纤维化相关基因的表达。结论 miR-207可能在肾脏纤维化中发挥重要的作用,为肾脏纤维化诊断靶点提供新的可能。
Objective To investigate the differential expression of miR-207 in urine samples and kidney of rat renal fibrosis（ renal fibrosis,RF） model and its function was investigated. Methods The rats were divided into two groups,including one undergoing ligation of the right ureter as UUO rat model,the other just under going the same surgery but no ligation for the ureters and as a shamed rat model. Real-time PCR（ RT-qPCR） was used to detect the expression of miR-207 in kidney and urine of rat renal fibrosis model. The function of miR-207 was investigated with transfection of miR-207 mimics and inhibitor to the rat renal tubular epithelial NRK-52 E cells. Results miR-207 expression in urine and kidney tissues of rat renal fibrosis model was upregulated（ P〈0. 05）. miR-207 increased the expression of gene related to fibrosis in rats renal tubular epithelial NRK-52 E cells. Conclusions miR-207 may play an important role in renal fibrosis and provide a potential method for the diagnosis of renal fibrosis.
出处
《基础医学与临床》
CSCD
2018年第3期308-311,共4页
Basic and Clinical Medicine
基金
国家重点基础研究发展计划(2013CB5308005)
