摘要
目的探讨BALB/c小鼠感染田鼠巴贝西虫后外周血中血小板活化情况及其与红细胞染虫率的关系。方法利用田鼠巴贝西虫红细胞染虫率为30%的种鼠抗凝血经腹腔注射感染BALB/c小鼠,感染后第1天开始采集BALB/c小鼠外周抗凝血,利用流式细胞术分别以白细胞分化抗原CD61(整合蛋白β3)和CD62P(P选择素)抗体标记,检测血小板和活化血小板数量;同时利用吉姆萨染色检测红细胞染虫率。采用Pearson相关性检验分析血小板活化率与红细胞染虫率的相关性。结果 BALB/c小鼠感染田鼠巴贝西虫后第2天其外周血中血小板的活化率开始升高,第3天最高,达(15.50±0.17)%,第4天开始下降,但无明显规律性;BALB/c小鼠在感染田鼠巴贝西虫后外周血中红细胞染虫率逐渐升高,第8天最高,达(63.10±3.43)%,之后逐渐下降。血小板活化率与红细胞染虫率比较差异无统计学意义(P=0.768)。结论田鼠巴贝西虫感染可引起宿主外周血中血小板的活化率升高,但与红细胞染虫率无显著相关性。
Objective To characterize platelet activation and its correlations with parasitemia in peripheral blood of miceinfected by Babesia microti. Methods BALB/c mice were intraperitoneally inoculated with B. microti infected blood,which was collected,heparinized, and diluted with sterile saline to achieve a 30% RBC parasitic infection rate. Followingthe infection,anti-coagulated peripheral blood was collected from the mice at each day. The blood was stained with anti-CD61 and anti-CD62 P antibodies, and then used to analyze the activation of platelet with flow cytometry. Furthermore, thepercentage of parasitized red blood cells was determined via Giemsa-stained thin blood films. Finally, Pearson correlationanalysis was used to evaluate the correlation of platelet activation and parasitemia. Results The percentage of plateletactivation started to increase on the second day post infection, reached the peak on the third day[（15.50±0.17）%], anddeclined from the fourth day, but was absence of obvious regularities. The percentage of parasitized red blood cellsgradually ascended post infection and reached the peak at the eighth day[（63.10±3.43）%], and then declined. However,there was no significant difference between the percentage of platelet activation and parasitized red blood cells, which wascharacterized by Pearson correlation analysis（P0.05）. Conclusion The platelet activation can be induced byB. microti infection, but it has no significant correlation with parasitemia.
出处
《中国媒介生物学及控制杂志》
CAS
2018年第1期34-37,共4页
Chinese Journal of Vector Biology and Control
基金
中国博士后科学基金(2015M580472)
江苏省自然科学基金(BK20150212)
徐州医科大学优秀人才科研启动基金(D2015006)~~
