摘要
目的通过Meta分析探讨中国人群EB病毒(EBV)感染与系统性红斑狼疮(SLE)相关性。方法计算机检索中国期刊全文数据库(CNKI)、维普中文科技期刊全文数据库(VIP),PubMed,EMbase,中国生物医学文献检索数据库(CBM)和万方科技期刊全文数据库,检索对中国人群SLE患者及对照血清中抗EB病毒抗体进行检测的病例-对照研究,检索时限均从建库至2017年7月。参照修改后的纽卡斯尔-渥太华量表(NOS)进行质量评价。采用Rev Man5.3和Stata12软件进行Meta分析,利用M-H法计算抗EB病毒抗体比值比(OR)值,采用敏感性分析评估结果的稳定性。利用Egger检验分析发表偏倚。结果最终纳入17项病例-对照研究,包括SLE患者1 392例,对照1 647例。对EB病毒衣壳抗原抗体(EBV-VCA)IgG进行检测的研究纳入12项,Meta分析结果显示SLE患者与健康对照EBV-VCA IgG阳性率差异有统计学意义(P=0.003),总体比值比OR为2.97(95%CI=1.43~6.15)。按SLE病例诊断方法、研究质量、病例与对照年龄是否匹配对其进行亚组分析后未发现任何亚组之间存在显著差异。Egger检验结果显示无显著发表偏倚。对EBV-VCA IgA,EBV-VCA IgM进行检测的研究分别纳入7项和6项。Meta分析结果显示EBV-VCA IgA,EBV-VCA IgM与SLE之间存在明显相关性(P<0.000 01),总体比值比OR分别为7.01(95%CI=4.61~10.66),9.34(95%CI=4.29~20.34)。但SLE患者EBV-VCA IgA(37.5%)和EBV-VCA IgM(28.5%)阳性率低于EBV-VCA IgG(90.3%)。SLE患者与健康对照核心抗原抗体(EBNA1)IgG阳性率差异无统计学意义(P=0.97),总体比值比OR为1.01(95%CI=0.61~1.67)。结论先前发生EB病毒感染与SLE之间存在明显相关性。
Objective To investigate the correlation between Epstein-Barr virus（EBV）infection and systemic lupus erythematosus（SLE）in Chinese population by meta-analysis.Methods The following databases such as China National Knowledge Infrastructure（CNKI）,Chinese Weipu Database（VIP）,PubMed,EMbase,Chinese Biological Medicine Database（CBM）,WANFANG Database were searched from inception to July 2017.For a study it had to be a case-control with SLE and controls in Chinese population.The study had to assay serum anti-EBV antibody in each group.Quality assessments were performed using a modified version of the Newcastle-Ottawa scale（NOS）.Then meta-analysis was performed using Rev Man 5.3 software and Stata 12.0 software.Mantel-Haenszel odds ratios（OR）for the detection of anti-EBV antibodies were calculated as well as stability evaluated by sensitivity analysis.Egger test was used to evaluate the publication bias.Results Seventeen case-control studies were included.These studies included a total of 1 392 SLE cases and 1 647 controls.Twelve studies measured anti-EBV viral capsid antigen（EBV-VCA）IgG,there was a statistically significant higher seroprevalence of anti-EBV-VCA IgG（P=0.003）in SLE cases compared to controls.Meta-analysis revealed an overall OR of2.97（95% CI=1.43~6.15）.Our subgroup analyses failed to demonstrate a significant difference between any of the subgroups considered.The Egger test showed that there was no significant publication bias.Seven studies measured anti-EBVVCA antibodies of the IgA and six studies assayed anti-EBV-VCA antibodies of the IgM subclass,Meta-analysis showed a significant association between anti-EBV-VCA IgA,anti-EBV-VCA IgM and SLE,with OR=7.01（95% CI=4.61~10.66）,9.34（95% CI=4.29~20.34）respectively.The percentage of SLE patients positive for anti-EBV-VCA IgA and anti-EBV-VCA IgM（37.5%and 28.5%,respectively）were significantly lower compared with anti-EBV-VCA IgG（90.3%）.Meta-analysis showed overall OR=1.01（95% CI=0.61~1.67）indicating tha
作者
张敏杰
张阳
徐丹
徐晓莉
ZHANG Min-jie;ZHANG Yang;XU Dan;XU Xiao-li(Department of Medical Laboratory, Xianyang Central Hospital, Shaanxi Xianyang 712000, China)
出处
《现代检验医学杂志》
CAS
2018年第1期25-31,共7页
Journal of Modern Laboratory Medicine
