摘要
利用光催化技术使大豆蛋白自组装形成纳米颗粒,并以此制备皮克林乳液。研究光催化时间对颗粒自组装形成过程的影响,并对乳液分别进行粒径分布、乳析指数、离心稳定性和贮藏稳定性的测定。结果表明,随着光催化时间的延长,蛋白颗粒的粒径增加,PDI值减小,疏水性增强,Z电位增大。当光催化时间超过30 min时,PDI值增大,疏水性减弱,Z电位降低。颗粒间主要以疏水作用和二硫键来维持光催化大豆蛋白颗粒稳定性。与大豆蛋白乳液相比,光催化大豆蛋白颗粒稳定的乳液表现出良好的贮藏稳定性和离心稳定性。利用激光共聚焦显微镜观察发现,皮克林乳液具有更高的界面蛋白吸附量和较厚的界面膜。光催化大豆蛋白颗粒有望成为食品级皮克林稳定剂的有效材料。
This work utilized nano particle assembled from soy protein by photo catalysis to stabilize Pickering emulsions. The effect of the photo catalysis time on self-assembly process of soy protein was investigated. Moreover, the particle size distribution, long-term storage stability, centrifugation stability, and creaming stability of Pickering emulsion were determined. The results showed that, increasing photo catalysis time resulted in a notable increase in droplet size and zeta potential, and improved the surface hydro phobicity, but reduced PDI slightly within 30 min. However, PDI was progressively increased by further increasing photo catalysis time, with a progressive but slight decline observed in surface hydro phobicity and zeta potential. The soy protein nano particle obtained by photo catalysis showed a strong integrity of structure that involved both hydro phobic interactions and disulfide bonds. Pickering emulsion showed good storage and centrifugation stability. By Confocal Laser Scanning Microscopy, it was observed that Pickering emulsion had higher surface protein absorption and a thicker interfacial membrane was formed around the oil droplets. Therefore, soy protein nano particle obtained by photo catalysis exhibit a great potential to be developed into a kind of effective foodgrade Pickering stabilizers.
作者
金蓓
官金敏
许佳音
廖凯琳
杨娟
Jin Bei;Guan Jinmin;Xu Jiayin;Liao Kailin;Yang Juan(School of Chemistry and Chemical Engineering, Lingnan Normal University, Zhanjiang 524048, Guangdon)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2018年第1期162-168,共7页
Journal of Chinese Institute Of Food Science and Technology
基金
国家自然科学基金项目(31601415)
关键词
大豆蛋白纳米颗粒
光催化
皮克林乳液
贮藏稳定性
soy protein nanoparticle
photocatalysis
Picketing emulsion
storage stability
