摘要
目的探索青藏高原野生田鼠是否可以作为泡球蚴感染的动物模型。方法将60只实验野生田鼠分为A组(皮下注射接种感染组)、B组(开腹肝穿刺注射感染组)、C组(经皮肝穿刺感染组),每组20只。感染3个月后观察各组大鼠的感染率、病死率。造模成功田鼠处死后分别从病灶浸润带、灶旁+50%病灶、病灶中心、液化部分、病灶边缘、病灶70%来进行HE染色,显微镜下观察包虫病灶的病理结构。从感染病灶中提取原头节,进行体外培养。计数资料比较采用χ~2检验。结果 A、B、C组田鼠泡球蚴感染的成功率分别为60%、80%、70%,3组比较差异无统计学意义(χ~2=4.138,P=0.126)。A、B、C组田鼠泡球蚴感染的病死率分别为5%、20%、15%,3组比较差异无统计学意义(χ~2=5.201,P=0.107)。将造模成功的田鼠病灶切片,从浸润带(灶旁2~3 mm)、灶旁+50%病灶、病灶中心(无液化部分)、液化部分、病灶边缘(不含正常组织)、病灶70%来进行HE染色,镜下可见被红染囊泡的角质层较薄、不连续,囊泡外可观察到大量炎性细胞浸润,所有切片中均未发现原头节。随后将病灶组织体外培养并染色,可发现大量存活原头节,不着色且有活动性。结论本研究证实高原野生田鼠作为青藏高原特有的物种,可以作为泡球蚴感染的动物模型,皮下接种感染泡球蚴是一种较合适的造模方法。
Objective To analyze whether Microtus fuscus in Qinghai-Tibet Plateau can be used as an animal model for Echinococcus infection.Methods Sixty wild voles（Microtus fuscus） were equally divided into groups A,B,and C to undergo Echinococcus inoculation through subcutaneous injection,direct transhepatic approach,and percutaneous transhepatic approach,respectively.Three months later,the infection rate and mortality rate were determined.After the model animals were sacrificed,HE staining was performed for the infiltration zone of lesion（2-3 mm near the lesion）,the area adjacent to the lesion and 50% of the lesion,the center of lesion（non-liquefied area）,the liquefied area of lesion,the margin of lesion（not including the normal tissue）,and 70% of the lesion,and the pathological structures of hydatid lesions were observed under a microscope.Protoscoleces were extracted from the lesions for in vitro culture.The chi-square test was used for comparison of categorical data.Results The success rate of Echinococcus infection in groups A,B,and C were 60%,80%,and70%,respectively,with no significant difference between the three groups（χ~2= 4.138,P = 0.126）.The mortality rates of groups A,B,and C were 5%,20%,and 15%,respectively,with no significant difference between the three groups（χ~2= 5.201,P = 0.107）.HE staining showed that the red-stained vesicles had thin,discontinuous cuticular layers,with massive inflammatory cell infiltration around the vesicles; no protoscoleces were observed in all sections.After in vitro culture and staining of the lesion tissue,a large number of live protoscoleces were observed,unstained and with motility.Conclusion This study confirms that Microtus fuscus,an endemic species in Qinghai-Tibet Plateau,can be used as an animal model for Echinococcus infection.Subcutaneous injection is an appropriate method for Echinococcus inoculation.
出处
《临床肝胆病杂志》
CAS
北大核心
2018年第2期373-377,共5页
Journal of Clinical Hepatology
基金
国家自然科学基金项目(81641078)
青海省科技厅自然科学青年基金项目(2016-ZJ-924Q)
青海大学附属医院中青年科研基金(ASRF-2014-07)
关键词
多房棘球绦虫
幼虫
田鼠亚科
青藏高原
ecbinococeus muhilocularis
larva
arvicolinae
Qinghai tibet plateau
