摘要
目的:采用APP/PS1双转基因AD模型小鼠通过两种行为学实验,观察补阳还五汤的抗阿尔茨海默病作用。方法:将60只APP/PS1小鼠随机分为5组,分别为模型组、多奈哌齐组、补阳还五汤高、中、低剂量组,以C57BL/6J作为空白组。给药30天后,开始进行行为学实验,包括新物体识别实验和避暗实验。用western blot方法检测小鼠海马炎症因子IL-6和TNF-α的表达。结果:与模型组相比,补阳还五汤高、中剂量组能够明显降低APP/PS1小鼠海马炎症因子IL-6和TNF-α的表达,并且能明显改善APP/PS1小鼠的学习记忆能力。结论:补阳还五汤能够明显降低APP/PS1双转基因AD模型小鼠炎症因子的表达,并且明显改善其学习行为能力,可能能够用于阿尔茨海默病的治疗。
This study was aimed to investigate the effects of Bu-Yang Huan-Wu（BYHW） decoction to the learning and memory ability of APP/PS1 double transgenic mice through two behavior tests, in order to observe the Alzheimer＇s disease（AD） treatment effect of BYHW decoction. A total of 60 APP/PS1 double transgenic mice were randomly divided into five groups, which were the model group, the hydrochloride group, the high-, middle-, and low-dose BYHW decoction group, with ten rats in each group. The C57 BL/6 J mice were used as the control group. After 30 days of drug administration, all mice were subjected to behavior tests, including new object recognition experiment and step-through task. Western blot was used to detect changes of inflammatory factors, including IL-6 and TNF-α, in hippocampus of mice. The results showed that compared with the model group, the high-and middle-dose BYHW decoction can significantly decrease inflammatory factors of IL-6 and TNF-α expression in hippocampus of APP/PS1 mice. It can also obviously improve the learning and memory ability of APP/PS1 mice. It was concluded that BYHW decoction can significantly decrease the inflammatory factor expression in APP/PS1 double transgenic AD model mice. It can obviously improve its learning and memory ability, which can be used in the treatment of AD.
出处
《世界科学技术-中医药现代化》
CSCD
2017年第11期1846-1850,共5页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家自然科学基金面上项目:基于雌激素/受体靶点效应的补肾中药治疗阿尔茨海默病的药效物质和作用机理研究(81673581)
负责人:张宁
哈尔滨市科技创新人才研究专项资金:骨碎补总黄酮治疗阿尔茨海默病的创新药物研究(2016RQYXJ012)
负责人:张宁
黑龙江中医药大学新药研究基金:骨碎补总黄酮治疗阿尔茨海默病的创新药物研究(2015xy04)
负责人:张宁
