摘要
目的:研究不同提取方法及给药途径的肠腑康胶囊对大鼠脾虚型溃疡性结肠炎(Ulcerative Uolitis,UC)的作用。方法:采用灌服番泻叶浸剂加直肠注入2,4,6-三硝基苯磺酸复制脾虚型溃疡性结肠炎模型,灌胃给药或者灌胃给药加直肠给药,观察其对脾虚型溃疡性结肠炎的治疗作用。结果:造模后各组大鼠陆续出现脾虚症状并有大鼠死亡;模型组结肠重量及体重结肠比显著升高,大鼠溃疡数增加,大鼠粪便异常的动物数显著增加,肠黏膜可见溃疡,可见"陷窝脓肿"、"陷窝溃疡"形成,炎症程度较重,可见肠黏膜和黏膜下组织大量炎细胞浸润。给药组给药后,症状明显得到改善,各给药组体重在给药10天及15天后,较造模组增长明显加快,肠腑康胶囊口服+直肠组结肠重量及体重结肠比显著降低;肠腑康胶囊分别提取组体重结肠比显著降低;肠腑康胶囊灌胃+直肠给药组和分别提取灌胃组肠内溃疡个数明显减少;肠腑康胶囊灌胃+直肠给药组和肠腑康胶囊分别提取灌胃组大鼠粪便异常的动物数显著减少。病理组织学检查:口服+直肠组、分别提取组、混合提取组、补脾益肠丸组可显著提高溃疡愈合评分,提示本品对溃疡性结肠炎有较好的治疗作用,肠腑康胶囊给药途径口服+直肠组的作用优于其他两个给药途径和提取方法。结论:肠腑康胶囊(4.8g/kg)对大鼠脾虚型溃疡性结肠炎有较好的治疗作用,不同给药途径和提取方法的肠腑康胶囊对大鼠脾虚型溃疡性结肠炎的影响不同,其中口服+直肠明显优于其他2个给药途径及方法。
Objective： To study therapeutic effects of Changfukang Capsule of different extraction methods and different feeding ways on mice ulcerative uolitis. Mothods： Drench infusion of senna leaf and rectal injection of 2,4,6-three nitrobenzene sulfonic acid was used to duplicate the model of ulcerative uolitis. By way of gastric or gastric plus rectal administration,its therapeutic effects on mice ulcerative uolitis was observed. Results： After establishment of model,spleen deficiency symptom gradually appears in all groups of mice,and there were some cases of death. Weight of segmented intestine and ratio of colon body weight in the model group significantly increased（ P〈0. 05,P〈0. 01）.The number of mice ulcer increased（ P〈0. 01）. The number of mice of fecal abnormality significantly increased（ P〈0. 05）. Ulcer of intestinal mucosa was observed. Lacunar abscess and Lacuna ulcer took place. The inflammation was severe. Large scale of inflammatory infiltration appeared in intestinal mucosa and submucosal tissue. Symptoms in drug group were obviously improved. 10-15 days after feeding,body weights in treated groups increased faster than that in the model group（ P〈0. 05,P〈0. 01）. Weights of segmented intestine and ratios of colon body weight in gastric plus rectal administration group decreased（ P〈0. 05,P〈0. 01）. Ulcer number of gastric plus rectal administration and separate extraction intragastric gavage group decreased（ P〈0. 05,P〈0. 01）. The number of mice of fecal abnormality in gastric plus rectal administration and separate extraction intragastric gavage groups significantly increased（ P〈0. 05）. In histopathologic examination,in gastric plus rectal administration group,separate extraction group,hybrid extraction group and Bupiyichang Wan positive group ulcer healing scores significantly increased（ P〈0. 01,P〈0. 01,P〈0. 05）,which indicated that this capsule had therapeutic effects on ulcerative uolitis. Gastric plus rectal administration had the better e
出处
《中药药理与临床》
CSCD
北大核心
2017年第6期114-117,共4页
Pharmacology and Clinics of Chinese Materia Medica
基金
陕西省科技统筹创新工程计划项目(2014KTCQ03-10)
关键词
肠腑康胶囊
脾虚型溃疡性结肠炎
Changfukang Capsule （ 肠腑康胶囊）
ulcerative uolitis
cytokines
