摘要
考察纤维素酶预处理对鼠尾草精油化学组分、抗氧化活性和抑菌效果的影响。结果表明,鼠尾草经酶预处理,精油得率提高了25.61%。通过气相色谱-质谱联用技术分析,共鉴定出29种成分。酶预处理得到的精油与对照组中含氧单萜类化物的含量分别为64.42%和53.28%。酶预处理能显著提高鼠尾草精油的还原力和DPPH自由基清除能力以及增强对两种革兰氏阳性细菌的抑制作用。纤维素酶预处理能提高精油的得率和生物活性,在食品和医药行业具有很大的应用前景。
The main goal of the present study is to evaluate the effects of cellulase pre-treatment on the yields,chemical composition,antioxidant and antimicrobial activities of the essential oils of Salvia officinalis L.The results showed that application of cellulase induced 25.61% increase in the essential oil yields.GC/MS analysis revealed the presence of 29 compounds in Salvia officinalis L.essential oil.Higher amounts of oxygenated monoterpenes were existed in the essential oil of enzymatic pre-treatment（64.42%） in comparison with control（53.28%）.Meanwhile,the antioxidant and antimicrobial activities were also assessed in vitro,cellulase pre-treatment extracted essential oil exhibited significantly stronger antioxidant ability of reducing power and radical scavenging activity of DPPH,and showed relatively good antimicrobial activity with the Diameter of inhibition zones,the minimum inhibitory concentration（MIC） and the minimal bactericidal concentration（MBC） values against two gram-positive bacteria.Cellulase pre-treatment could be useful for enhancing yield and biological activities of the essential oils,and hold a good potential for use in food and pharmaceutical industries.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2017年第10期145-151,共7页
Journal of Chinese Institute Of Food Science and Technology
基金
国家自然科学基金项目(31301580)
浙江省自然科学基金项目(LY13C200012)
关键词
纤维素酶
鼠尾草
精油
化学组分
抗氧化活性
抑菌活性
cellulase
Salvia officinalis L.
essential oil
chemical composition
antioxidant activity
antimicrobial activity
