摘要
为了探讨蜂王浆主蛋白对小鼠抗疲劳能力的作用及其生理机制,通过给小鼠灌胃低、中、高3个剂量组蜂王浆主蛋白,同时设立对照组,观察小鼠体重、脏器系数、负重游泳时间、常压和急性耐缺氧时间、肝糖原和肌糖原含量、血乳酸、乳酸脱氢酶、尿素氮等指标的变化。结果表明:蜂王浆主蛋白能延长小鼠负重游泳时间,显著增加小鼠肝糖原和肌糖原含量,减少小鼠运动后血乳酸含量,减少小鼠运动后尿素氮的积累。此外,在常压耐缺氧实验中,与空白对照组相比,蜂王浆主蛋白高剂量组可显著延长小鼠在密闭缺氧条件下的存活时间;在急性脑缺血性缺氧实验中,各实验组张口喘气时间均长于对照组。蜂王浆主蛋白对小鼠具有显著的抗疲劳的作用。
The aim of the present study was to investigate the anti-fatigue effect of MRJPs in mice and the physiological and molecular mechanisms underlying this effect.Mice were randomly divided into five groups and three of the groups were administered different doses of MRJPs.Four weeks after MRJPs was administrated gavage to mice at three different doses,the anti-fatigue effect of MRJPs was evaluated by the weight-loaded swimming test and biochemical parameters related to fatigue,such as serum urea nitrogen（SUN）,lactic dehydrogenase（LDH）,blood lactic acid（LA） and hepatic and muscle glycogen.Otherwise,We carry out the hypoxia tolerance experiment and acute cerebral ischemia hypoxia experiment.The results showed that compared with the negative control group,the swimming times of the low-,medium-and high-dose groups were longer than those of the control group.Examinations revealed that the hepatic and muscle glycogen,lactate dehydrogenase and blood glucose concentration levels of the treatment groups were higher than those of the control group（P0.05）.The survival time of all test groups were longer than the control group in the survival experiment of normobaric hypoxia test and in acute cerebral ischemia hypoxia experiment,respectively.The results of the present study indicated that MRJPs exerts an anti-fatigue effect on mice.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2017年第10期23-29,共7页
Journal of Chinese Institute Of Food Science and Technology
基金
江西省科技支撑计划项目(20121112040007)
