摘要
目的:探索新疆维吾尔族糖耐量正常人群及2型糖尿病患者粪样中具核梭杆菌水平与2型糖尿病的相关性。方法:收集新疆维吾尔族糖耐量正常人群(NGT)及2型糖尿病(T2DM)患者血样和粪便样本,采用16SrDNA实时荧光定量PCR技术检测粪便样本中具核梭杆菌水平;运用Pearson相关分析方法分析该菌水平与两组人群的体质量指数(BMI)、空腹血糖(FBG)、胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)水平的相关性。结果:与维吾尔族糖耐量正常组比较,该民族T2DM组中具核梭杆菌水平显著升高(P=0.011)。具核梭杆菌水平与BMI(r=0.335,P=0.049)、TC(r=0.472,P=0.011)、LDL(r=0.368,P=0.029)水平呈正相关。结论:新疆维吾尔族T2DM患者肠道中具核梭杆菌水平可能与该民族T2DM患者的糖脂代谢紊乱密切相关。
AIM： To detect the levels of Fusobacterium nucleatum in Uygurs of Xinjiang with normal glucose tolerance（ NGT） or type 2 diabetes mellitus（ T2DM） and to analyze the correlation between Fusobacterium nucleatum level and type 2 diabetes mellitus.METHODS： Blood and fecal samples were collected from Uygurs of Xinjiang with NGT or T2DM respectively.16 SrDNA quantitative real-time PCR technology was used to detect the levels of Fusobacterium nucleatum in fecal samples.The correlations between Fusobacterium nucleatum and BMI,TC,TG,HDL and LDL levels were analyzed by Pearson.RESULTS：（ 1） The levels of Fusobacterium nucleatum in Uygurs T2DM group were significantly higher than those in Uygurs NGT group（ P = 0.011）.（ 2） Fusobacterium nucleatum was positively correlated with the levels of BMI（ r =0.335,P = 0.049）,TC（ r = 0.472,P = 0.011）and LDL（ r = 0.368, P = 0.029）.CONCLUSION： The levels of Fusobacterium nucleatum may be closely related to lipid metabolism of T2DM in Uygurs.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2017年第12期1321-1325,共5页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
国家自然科学基金联合基金优青项目(U1403322)
关键词
具核梭杆菌
2型糖尿病
实时荧光定量PCR
Fusobacterium nucleatum
type 2diabetes mellitus
quantitative real-time PCR
