摘要
目的建立富硒食用菌中硒代胱氨酸(Se Cys)、4价硒(Se(IV))、硒代蛋氨酸(Se Met)、6价硒(Se(VI))4种硒形态的高效液相色谱-电感耦合等离子体质谱(high performance liquid chromatography-inductively coupled plasma-mass spectrometry,HPLC-ICP-MS)的分析方法。方法以Hamilton PRP-X100为色谱分离柱,以40mmol/L磷酸氢二铵(pH 6.0)为流动相,对富硒食用菌中4种硒形态进行分离,利用电感耦合等离子体质谱法对质量数为77的硒同位素(~77Se)进行定量检测,样品提取采用蛋白酶(来源于地衣芽孢杆菌)为提取剂,60℃水浴提取1 h。结果 4种硒形态在0~100μg/L浓度范围内线性良好,相关系数r^2大于0.997,检出限在2μg/L以下。结论该方法适用于富硒食用菌中硒的形态分析。
Objective To establish a method for determination of 4 selenium species（Se Cys, Se（IV）, Se Met, Se（VI）） in selenized edible fungi using high performance liquid chromatography-inductively coupled plasma-mass spectrometry（HPLC-ICP-MS）. Methods An Hamilton PRP-X100 column was used to separate the 4 compounds using 40 mmol/L ammonium dibasic phosphate（pH 6.0） as the mobile phase, and the contents of isotope with a mass of 77 of these 4 compounds were determined by plasma mass spectrometry. The 4 selenium compounds were effectively extracted using protease（from Bacillus licheniformis） with water bath for 60 min. Results Four kinds of selenium compounds had good linear relationships in the range of 0~100 μg/L, and the correlation coefficients were larger than 0.997. The limits of detection were less than 2 μg/L. Conclusion The method is suitable for the simultaneous determination of selenium compounds in selenized edible fungi.
出处
《食品安全质量检测学报》
CAS
2017年第12期4688-4693,共6页
Journal of Food Safety and Quality
基金
湖南省食药监局食品药品安全科技项目(R201609)~~
关键词
高效液相色谱-电感耦合等离子体质谱
富硒食用菌
硒
形态分析
high performance liquid chromatography-inductively coupled plasma-mass spectrometry
selenizededible fungi
selenium
speciation analysis
