摘要
目的建立鲜三七花、茎、叶、根中7种人参皂甙成分分析的超高效液相色谱测定方法,并测定云南文山产鲜三七花、茎、叶、根中7种人参皂甙成分。方法样品粉碎后用水超声提取,提取液离心,取上清液通过Amberlite-XAD大孔树脂,用甲醇洗脱后蒸干,再用初始流动相定容,上机分析。采用二极管阵列检测器(diode array detector,DAD)检测,外标法定量。结果在14 min内完成7种目标化合物的分离分析,人参皂甙Rg1、Re、Rb1、Rc、Rb2、Rb3和Rd分别在0.0240～0.6005、0.0198～0.4960、0.0272～0.6810、0.0222～0.5545、0.0199～0.4974、0.0202～0.5055和0.0216～0.5395μg范围内呈良好的线性关系,各组分回收率范围为78.5%～95.4%,RSD为0.55%～0.77%(n=6)。结论该方法灵敏、快速、准确,可用于云南文山产鲜三七花、茎、叶、根中人参皂甙成分的检测。
Objective To establish a method for the determination of ginsenosides of different parts of fresh Panax Notoginsengs by ultra performance liquid chromatography（UPLC）, and determine the content of 7 kinds of ginsenosides in flowers, stems, leaves and roots of fresh Panax Notoginsengs from Wenshan, Yunnan province. Methods Sample were pulverized with water ultrasonic extraction, the extraction liquid was centrifuged, the cleaning liquid was removed by Amberlite-XAD large hole resin and then evaporated with methanol, then the initial flow phase was determined and analyzed by the instrument. The diode array detector and the external standard method were used. Results The separation of 7 target compounds had finished within 14 minutes. The 7 kinds of ginsenosides Rg1, Re, Rb1, Rc, Rb2, Rb3 and Rd had good linear relationships in the ranges of 0.0240～0.6005, 0.0198～0.4960, 0.0272～0.6810, 0.0222～0.5545, 0.0199～0.4974, 0.0202～0.5055, and 0.0216～0.5395 μg, respectively. The recovery ranges of the components were 78.5%～95.4%, with the relative standard deviations（RSDs） of 0.55%～0.77%（n=6）. Conclusion The method is sensitive, rapid and accurate, which can be used in the detection of ginsenosides in flowers, stems, leaves and roots of fresh Panax Notoginsengs from Wenshan, Yunnan province.
出处
《食品安全质量检测学报》
CAS
2017年第10期3864-3869,共6页
Journal of Food Safety and Quality
关键词
三七
超高效液相色谱
人参皂甙
Panax Notoginsengs
ultra performance liquid chromatography
ginsenosides
