摘要
目的观察不同程度颅脑创伤(TBI)大鼠外周循环血中内皮祖细胞(EPCs)和认知能力的动态变化。方法采用随机数字表法将28只雄性SD大鼠分为假手术组和轻、中、重度TBI组,每组7只。在前囟后4 mm,矢状缝右侧旁开3 mm处(海马区域)开圆形骨窗,轻、中、重度TBI组分别采用0.9、2.1、3.2 atm(1 atm=101.325 kPa)的力度打击骨窗建立TBI模型,分别在创伤前(0 h)及创伤后3、6、24、48、72、168、240、336 h取内眦球后静脉丛血,检测其循环血中EPCs水平,并进行白细胞(WBC)和血小板(PLT)计数。TBI后第21~25天,采用Morris水迷宫定位巡航实验和空间探索实验比较各组大鼠的逃避潜伏期和目标象限百分率。结果假手术组循环血中EPCs数量在整个实验过程中保持稳定。TBI后3 h,轻、中、重度TBI组循环血中的EPCs数量[(17.4±3.1)个、(15.6±5.0)个、(23.6±3.0)个]低于假手术组[(53.6±7.9)个],差异有统计学意义(P<0.05);在伤后6 h,EPCs数量迅速升高,且轻度、中度TBI组高于假手术组(P<0.05),约48 h后降至正常水平。整个实验过程中EPCs与WBC、PLT的变化并不一致。定位巡航实验结果显示各组大鼠逃避潜伏期随着创伤时间的延长而缩短;同期各TBI组逃避潜伏期均较假手术组延长。空间探索实验结果显示,中、重度TBI组大鼠停留在目标象限百分率明显低于假手术组和轻度TBI组。结论颅脑创伤大鼠随着创伤程度加重,其认知能力降低,且循环血中EPCs的水平与病情严重程度有关,可作为判断预后的标志物。
Objective To observe the dynamic changes of endothelial progenitor cells(EPCs) in peripheral blood andcognitive ability of rats with different degrees of traumatic brain injury(TBI). Methods A total of 28 male SD rats wererandomly divided into 4 groups: sham group, mild traumatic brain injury group, moderate traumatic brain injury group andsevere traumatic brain injury group, seven rats in each group. A hole was drilled on the right parietal skull(4.0 mm posteriorfrom bregma and 3 mm lateral to the sagittal suture, hippocampal region) to expose the dura. Rats were subjected to differentdegrees of traumatic brain injury of 0.9, 2.1, 3.2 atm(1 atm=101.325 k Pa). The dynamic changes of EPCs, white blood cellcount(WBC) and platelets(PLT) in the circulating blood were measured before(0 h) and after TBI(3, 6, 24, 48, 72, 168, 240 and 336 h after trauma). Morris water maze(MWM) test was performed to record the escape latency and target quadrantchange on day 21-25 after TBI in four groups. Results The number of circulating EPCs kept stable throughout theexperiment in the sham group. The numbers of EPCs were significantly lower at 3 h after injury in mild, moderate and severetraumatic brain injury groups(17.4±3.1, 15.6±5.0 and 23.6±3.0) than those in the sham group(53.6±7.9, P<0.05). Thenumbers of EPCs at 6 h after injury were increased rapidly, and which were significantly higher in the mild and moderate TBI group than those in sham group(P<0.05). Then the number of EPCs dropped to the normal level on 48 h after injury. Thechanges of EPCs was inconsistent with the WBC and PLT during the whole experiment. The positioning cruise experiment showed that the escape latency shortened over time in each group. The escape latency was longer in TBI group than that insham group during the same period. The spatial probe test showed that the percentages of the target quadrant weresignificantly lower in the moderate and severe TBI groups than those in the sham group and the mild TBI group.Conclusion With the severity of traumatic
出处
《天津医药》
CAS
北大核心
2018年第2期139-143,共5页
Tianjin Medical Journal
基金
国家自然科学基金面上项目(81671380)
天津市自然科学基金项目(17JCZDJC35900)
天津市应用基础与前沿技术研究计划项目(15JCQNJC11300)
天津市高等学校基本科研业务费资助项目重点项目(2016YD02)
