摘要
目的分析Linc00152在泛癌不同组织中表达差异及其表达情况与肿瘤的恶性程度及患者预后的关系,并探究Linc00152在不同类型癌细胞中相关功能。方法选取33例乳腺标本进行RNA-Seq,用q PCR方法检测50对乳腺癌组织和正常组织Linc00152的表达,结合GEO和TCGA数据库,分析Linc00152表达与乳腺癌恶性程度及患者预后的相关性;分析Linc00152在不同类型癌症、不同分期和分级中的表达情况。采用MDA-MB-231乳腺癌细胞系、SGC-7901胃癌细胞系、786-O肾癌细胞系进行细胞增殖能力、细胞凋亡和细胞迁移侵袭能力检测。结果 Linc00152在乳腺癌组织高表达(P<0.001),在HER2阳性和三阴性乳腺癌中Linc00152表达更高(P<0.0001);Linc00152高表达的乳腺癌患者无事件生存期和无转移生存期较差(P<0.001;P<0.01);敲降Linc00152后细胞增殖、迁移、侵袭能力降低,细胞凋亡增加(P<0.05)。结论 Linc00152在恶性肿瘤中具有促癌作用,可能是潜在的治疗靶点。
Objective The aim of this study was to explore the expression,prognosis and related function of Linc00152 in breast cancer. Methods Thirty-three cases of breast specimens were selected for RNA-Sequencing. The expression of Linc00152 was detected by qPCR in 50 pairs of breast cancer tissues and adjacent tissues. Combined with GEO and TCGA databases,the correlation between Linc00152 expression and the degree of malignancy and the prognosis of patients was analyzed. Cell proliferation,apoptosis and cell migration were detected in breast cancer MDA-MB-231 cell line,gastric cancer SGC-7901 cell line and renal cell carcinoma 786-O cell line. Results Linc00152 was highly expressed in breast cancer（ P〈0. 001）,and was higher in HER2 positive and triple negative breast cancer（ P〈0. 001）. In patients with high expression of Linc00152,the event-free survival and the metastasis-free survival were very poor（ P〈0. 001,P〈0. 01）. After knockdown Linc00152,the cell proliferation,migration and invasion were decreased and the apoptosis was increased（ P〈0. 05）. Conclusion Linc00152 has a role of promoting cancer in malignant tumors and may be a potential therapeutic target.
出处
《实用肿瘤学杂志》
CAS
2018年第1期7-13,共7页
Practical Oncology Journal
基金
国家自然科学基金(81602323)
