摘要
目的采用大鼠腹主动脉-髂总动脉建立适于神经外科医师进行显微血管吻合综合训练的动物模型。方法取成年清洁级SD大鼠12只,全身麻醉后开腹,显露并充分游离腹主动脉及双侧髂总动脉,测量腹主动脉、髂总动脉长度及各节段管径。选择无分支动脉发出且较长节段的腹主动脉主干行端-端血管吻合术,一侧髂总动脉和骶正中动脉进行端-侧吻合,双侧髂总动脉行端-侧及侧-侧吻合术。采用微型彩色多普勒超声探头检查各吻合口的血流通畅情况。结果对12只SD大鼠成功进行腹主动脉-髂总动脉的解剖分离,每只大鼠可供完成4次血管吻合练习。在左肾动脉下方至右侧髂腰动脉之间的腹主动脉主干长度(15.6±2.5)mm,管径(1.6±0.2)mm,行端-端吻合;骶正中动脉平均管径(0.80±0.08)mm,做"鱼嘴"状切开后,与右侧髂总动脉行端-侧吻合。左侧髂总动脉长(9.0±1.5)mm,管径(1.0±0.1)mm,在其发出处切断,与右侧髂总动脉行端-侧吻合;对双侧髂总动脉行端-侧吻合后,其远段并行排列有(5.1±0.3)mm的长度可行侧-侧吻合。结论大鼠腹主动脉-髂总动脉模型可较为全面地用于模拟神经外科常用的血管旁路移植术式,适于有一定显微外科技术基础的神经外科医师进行初步血管吻合训练。
Objective To establish an animal model suitable for neurosurgeons for the comprehensive training of microvascular anastomosis using rat abdominal aorta and common iliac artery. Methods Twelve adult SD rats were selected,they were generally anesthetized and laparotomized. The abdominal aortas and bilateral common iliac arteries were exposed and fully separated. The lengths and diameters of abdominal aortas and common iliac arteries of each segment were measured. The end-to-end anastomosis were performed choosing the main trunk of the abdominal aorta without a branching artery and longer segment. The unilateral common iliac artery and the sacral median artery were used for end-to-side anastomosis. The bilateral common iliac arteries were used for end-to-side and side-to-side anastomosis. The micro Doppler ultrasound probe was used to detect the blood flow patency of each anastomotic stoma. Results Anatomical separation of the abdominal aortas and the common iliac arteries was successfully performed in 12 SD rats. Each rat could provide 4 vascular anastomosis exercises. The length of abdomen aorta trunk was 15. 6 ± 2. 5 mm and the diameter was 1. 6 ± 0. 2 mm between the lower left renal artery and right iliolumbar artery. The side-toside anastomosis was performed. The mean diameter of the median sacral arteries was 0. 80 ± 0. 08 mm. After the fish mouth-like cutting,the end-to-side anastomosis of the right common iliac arteries were conducted. The lenth of left common iliac artery was 9. 0 ± 1. 5 mm,the diameter was 1. 0 ± 0. 1 mm,and was cut off at its origin and then the end-to-side anastomosis of the right common iliac arteries were conducted. After end-toside anastomosis of bilateral common iliac arteries,its distal segment was arranged in parallel with a length of 5. 1 ± 0. 3 mm,and then the side-to-side anastomosis could be conducted. Conclusions The rat abdominal aorta and iliac artery model can be comprehensively used to simulate the commonly used neurosurgery bypass graft. It is suitable for neurosurg
出处
《中国脑血管病杂志》
CAS
CSCD
北大核心
2018年第2期77-82,共6页
Chinese Journal of Cerebrovascular Diseases
基金
国家十三五科技支撑计划(2015BAI12B00)
