摘要
目的探讨广西地区由Fonsecaea monophora所致着色芽生菌病患者的实验室和临床资料特点。方法取患者患处皮损组织进行真菌镜检、真菌培养、组织病理学检查,利用引物ITS1、ITS4将培养出的菌株进行r DNA ITS区序列的PCR扩增,扩增产物测序后在Gen Bank核酸数据库中进行同源性比对。结果直接镜检可见多个圆形、厚壁、棕色硬壳细胞,真菌培养可见暗棕色,橄榄色至黑色菌落生长,皮损组织病理表现为慢性肉芽肿样改变,并可见硬壳小体。ITS区序列分析鉴定为Fonsecaea monophora。依据临床及实验室检查均确诊为Fonsecaea monophora所致的着色芽生菌病。口服伊曲康唑200mg,2次/d治疗有效。结论研究首次发现Fonsecaea monophora是我国广西地区着色芽生菌病的重要病原菌,ITS区序列分析是该菌种鉴定的重要手段。
Objective To investigate the laboratory and clinical data characteristics of the patients with chromoblastomycosis caused by Fonsecaea monophora in Guangxi, China. Methods Patient's skin lesions were used for fungal microscopy, fungal culture, and histopathological examination. PCR was used to amplify the DNA of the pathogens with primers ITS1 and ITS4. The sequences of ITS1 and ITS4 were identified using GenBank. Results Direct microscopic examination showed multiple round cells, thick wall, brown muriform cells. Fungal culture showed dark brown, and olive to black colonies. Histopathologic examination demonstrated chronic granuloma and sclerotic bodies. ITS sequence analysis identified Fonsecaea monophora. Treatment with 200mg of Itraconazole twice-daily was effective. Conclusion For the first time we find that Fonsecaea monophora is one of the most important pathoden of chromoblastomycosis in Guangxi, and ITS sequence analysis is an effective identification method.
出处
《中国皮肤性病学杂志》
CAS
CSCD
北大核心
2018年第3期287-290,共4页
The Chinese Journal of Dermatovenereology
基金
国家自然基金(03101215003D)
百科计(20150825)