摘要
为明确转cry1Ab+cry1C双价抗虫水稻对二化螟的抗性及其杀虫蛋白的时空表达,本研究采用离体水稻组织生测法系统评价了cry1Ab、cry1C、正交cry1Ab+cry1C和反交cry1C+cry1Ab 4种抗虫水稻品系对二化螟的杀虫效果,并用ELISA方法测定了Cry1Ab、Cry1C蛋白在各个抗虫水稻品系中的表达情况。结果显示,二化螟在不同生育期的4种转基因抗虫水稻上取食的叶面积显著低于非转基因对照亲本明恢63。4个转基因抗虫水稻品系在生长前期(苗期、分蘖和拔节期)对二化螟表现极高的杀虫效果,生长后期(孕穗期和成熟期)杀虫效果有所下降。两双价抗虫水稻杀虫效果最好,其次为转cry1Ab和cry1C水稻。Bt蛋白表达水平随着抗虫水稻生育期的变化而变化,且差异显著;Cry1Ab的蛋白表达量在水稻整个生长期均显著高于Cry1C。Cry1Ab蛋白在单价抗虫水稻叶片和茎杆中均表现出随生育期先升高后下降的趋势,但在双价抗虫水稻中表现出随生育期逐渐下降的趋势。Cry1C蛋白在单、双价抗虫水稻的叶片组织中均表现出逐渐升高的趋势。与单价抗虫水稻相比,双价抗虫水稻中的Cry1Ab、Cry1C的蛋白表达水平并没有表现出显著的降低。因此,双价抗虫水稻不仅能高效防治害虫,还能延缓害虫抗性,在生产上表现出良好的应用前景。该研究结果可望为转Bt基因抗虫作物的环境安全评价提供科学数据和理论依据。
Control efficacies of four transgenic rice lines,cry1Ab,cry1C,cry1Ab＋cry1C and cry1C＋cry1Ab,against the main target pest,Chilo suppressalis,were evaluated through detached rice tissues bioassay,and Cry1Ab and Cry1C protein contents in these transgenic lines were measured by enzyme-linked immunosorbent assays.The results showed cumulative feeding areas of C.suppressalis feeding on transgenic rice lines for 48 h and 96 h were signif icantly lower than those on non-transgenic rice line,MH63.Four insect-resistant transgenic rice lines presented very high resistant level against C.suppressalis at early developmental stage（seedling,tillering and elongat ion stages）,while control efficacy of transgenic lines obviously decreased at later growth stages（booting and maturing stages）.Double insect-resistant genes pyramided rice lines exhibited the highest resistant level among the four transgenic lines,second followed by cry1Ab and cry1C lines.Both Cry1Ab and Cry1C protein levels showed signif icantly different in respective transgenic rice lines at five different developmental stages.Addit ively,Cry1Ab protein expressing levels in transgenic lines were signif icantly higher than Cry1C protein.Cry1Ab levels were shown firstly increased and then decreased with the increase of growth stages in single-gene line,however,they presented the gradually decreasing trend with the increasing developmental stages both in leaf and stem tissues indouble-gene lines.Cry1C levels showed the gradually rising trend with the increasing developmental stages in leaf tissues both in single-and double-gene lines.Moreover,Cry1Ab or Cry1C levels did not exhibit the signif icant decrease in double-gene lines,when compared with that in single-gene lines.Therefore,two-gene pyramided plants have robust prospects for commercial use,because they not only provide the efficient control against the pests,but also delay the insect resistance evolution.These results will provide the scientific data and theoretical basis for environmental safety asse
作者
陈耕
何珊
韩兰芝
彭于发
CHEN Geng;HE Shan;HAN Lanzhi;PENG Yufa(State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China)
出处
《中国生物防治学报》
CSCD
北大核心
2018年第1期71-78,共8页
Chinese Journal of Biological Control
基金
国家转基因生物新品种培育重大专项(2016ZX08011-001)
国家自然科学基金(31572336)
关键词
二化螟
转基因抗虫水稻
抗虫效率
杀虫蛋白表达
Chilo suppressalis
insect-resistant transgenic rice
control efficacy
insecticidal protein expression
