摘要
为了解西南山地板栗的主要害虫二斑栗实象各地理种群的遗传变异及基因交流情况,对采自于云南省9个地理种群的二斑栗实象线粒体COI基因序列进行测序,并根据其结果进行种群间分子变异、单倍型、遗传多样性、遗传分化程度和基因流分析。结果表明:获得的79条COI基因序列大小为693 bp,检测到多态性位点32个,形成单倍型34种,其中一种为所有地理种群所共享;总群体单倍型多样性Hd为0.901 01,固定系数Fst为0.132 82,遗传分化系数Gst为0.052 55,基因流Nm为9.01。总种群间及各种群Tajima's D检验结果均不显著,说明二斑栗实象种群数量较为稳定未出现种群扩张。二斑栗实象的遗传分化主要来自种群内部,地理种群间的遗传距离与地理距离之间有一定的相关性;各地理种群未形成明显的地理结构,说明种群基因交流频繁。
Abstract：This study aimed at investigating the genetic differentiation and gene flow among geographic populations of Curculio bimaculatus. The partial sequences of mtDNA COI gene in 9 geographic populations of Cbimaculatus were sequenced and analyzed in Yunnan. The molecular variance, the haplotype differentiation, the genetic diversity, gene flow were analyzed. A total of 79 COI gene with 32 variable sites and 34 haplotypes were obtained and were 693bp, including 1 haplotype shared by 9 population. The haplotype diversity index （Hd） was 090101, the total fixztion index （Fst） was 013282, the total genetic differentiation coefficient （Gst） was 005255 and the gene flow （Nm） was 901. The Tajima′s D test for all populations or each single population of Cbimaculatus did not reach any significant level, which suggest that there might not have been a recent population expansion and population stability. The results of AMOVA analysis demonstrated that genetic differentiation of Cbimaculatus mainly occurred within populations. The Mantel test disclosed that the genetic distance among populations was correlated with the geographic distance. The haplotype network and neighborjoinjng phylogenetic tree showed that the haplotypes are distributed in different clades and no obvious geographical structure has been formed. This indicates that the high level of gene exchange did exist in different geographical populations.
出处
《西南林业大学学报（自然科学）》
CAS
北大核心
2018年第1期110-116,共7页
Journal of Southwest Forestry University：Natural Sciences
基金
云南省教育厅项目(2015Y283)资助
云南省中青年学术和技术带头人后备人才项目(2014HB020)资助
关键词
二斑栗实象
线粒体
COI基因
地理种群
遗传分化
基因流
Curculio bimaculatus
mitochondria
mtDNA CO1 gene
geographic population
genetic differentiation
gene flow
